TriMethyl-Histone H3-K27 mAb

Signal Transduction

WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200

IP: 1:50 - 1:200

ChIP: 1:20 - 1:100

Monoclonal

Unconjugated

Store at -20˚ C. Avoid freeze / thaw cycles.

Methylated

8290

H3K27me3

Homo sapiens

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K27 antibody (16-486) at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded mouse brain using TriMethyl-Histone H3-K27 antibody (16-486) at dilution of 1:25 (40x lens).

Immunofluorescence analysis of C6 cells using TriMethyl-Histone H3-K27 antibody (16-486) at dilution of 1:25. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2OS cells using TriMethyl-Histone H3-K27 antibody (16-486) at dilution of 1:25. Blue: DAPI for nuclear staining.