BAX Antibody, KO Validated

blue ice or RT

A synthetic peptide of human BAX

Raji

HepG2

Mouse testis

Antibody is Knockout validated.

Polyclonal

Unconjugated

Store at -20˚ C. Avoid freeze / thaw cycles.

None

581

BAX

Homo sapiens

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.

Western blot analysis of extracts from normal (control) and BAX knockout (KO) 293T cells, using BAX antibody (16-152) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.