ArtNr |
PRS-14-831-50uL |
Hersteller |
ProSci
|
Menge |
50 uL |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Liquid |
Applikationen |
WB, IHC |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Splicing factor U2AF 35 kDa subunit, U2 auxiliary factor 35 kDa subunit, U2 small nuclear RNA auxiliary factor 1, U2 snRNP auxiliary factor small subunit, U2AF1, U2AF35, U2AFBP |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Apoptosis, Cell Cycle |
Immunogen |
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human U2AF1 (NP_006749.1). |
Applications |
WB: 1:500 - 1:2000
IHC: 1:100 - 1:200 |
Positive Control 1 |
293T |
Positive Control 2 |
HeLa |
Positive Control 3 |
K-562 |
Positive Control 4 |
LO2 |
Positive Control 5 |
Mouse spleen |
Positive Control 6 |
Mouse kidney |
Predicted Molecular Weight |
Observed: 38kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
U2AF1 |
Ncbi Gene Id # |
7307 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
U2AF1 |
Ncbi Official Full Name |
Splicing factor U2AF 35 kDa subunit |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q01081 |
Background |
This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using U2AF1 antibody (14-831) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.