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EP300 Antibody

EP300 Antibody

ArtNr	PRS-14-746-50uL
Hersteller	ProSci
Menge	50 uL
Kategorie	Oncology Antibodies By Cell Type Carcinoma Hematology Leukemia Lymphoma Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies
Typ	Antibody Primary
Format	Liquid
Applikationen	WB, IF, IHC
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	EP300, E1A binding protein p300, Histone acetyltransferase p300, KAT3B, p300 HAT, E1A-associated protein p300, E1A-binding protein, 300kD, p300, RSTS2
Lieferbar
Shipping	blue ice or RT
By Research Area	Apoptosis, Cancer, Cell Cycle, Immunology, Neuroscience, Obesity, Signal Transduction, Stem Cell, Transcription
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human EP300 (NP_001420.2).
Applications	WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IF: 1:50 - 1:200
Positive Control 1	Mouse brain
Positive Control 2	Rat brain
Predicted Molecular Weight	Observed: 280kDa
Purification	Affinity purification
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions	Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	EP300
Ncbi Gene Id #	2033
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	EP300
Ncbi Official Full Name	E1A binding protein p300
Ncbi Organism	Homo sapiens
Swissprot #	Q09472
Background	This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer.
1st Image Caption	Western blot analysis of extracts of various cell lines, using EP300 antibody (14-746) at 1:3000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.
2nd Image Caption	Immunohistochemistry of paraffin-embedded rat ovary using EP300 antibody (14-746) at dilution of 1:100 (40x lens).
3rd Image Caption	Immunohistochemistry of paraffin-embedded rat brain using EP300 antibody (14-746) at dilution of 1:100 (40x lens).
4th Image Caption	Immunohistochemistry of paraffin-embedded human lung cancer using EP300 antibody (14-746) at dilution of 1:100 (40x lens).
5th Image Caption	Immunohistochemistry of paraffin-embedded human breast using EP300 antibody (14-746) at dilution of 1:100 (40x lens).
6th Image Caption	Immunohistochemistry of paraffin-embedded mouse brain using EP300 antibody (14-746) at dilution of 1:100 (40x lens).

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 50 uL

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Lieferung vsl. bis 04.10.2024

Express-Lieferung: 7 Tage sparen

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