Vergleich

RPL32 Antibody

ArtNr PRS-14-738-50uL
Hersteller ProSci
Menge 50 uL
Kategorie
Typ Antibody Primary
Format Liquid
Applikationen WB, IF, IHC
Specific against Human, Mouse, Rat
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias RPL32, L32, PP9932
Lieferbar
Shipping
blue ice or RT
By Research Area
Other
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human RPL32 (NP_000985.1).
Applications
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Positive Control 1
A-549
Positive Control 2
HepG2
Positive Control 3
NIH/3T3
Positive Control 4
Mouse liver
Positive Control 5
Mouse stomach
Positive Control 6
Mouse spleen
Predicted Molecular Weight
Observed: 16kDa
Purification
Affinity purification
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions
Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
RPL32
Ncbi Gene Id #
6161
User Note
Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol
RPL32
Ncbi Official Full Name
ribosomal protein L32
Ncbi Organism
Homo sapiens
Swissprot #
P62910
Background
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L32E family of ribosomal proteins. It is located in the cytoplasm. Although some studies have mapped this gene to 3q13.3-q21, it is believed to map to 3p25-p24. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternatively spliced transcript variants encoding the same protein have been observed for this gene.
1st Image Caption
Western blot analysis of extracts of various cell lines, using RPL32 antibody (14-738) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
2nd Image Caption
Immunofluorescence analysis of L929 cells using RPL32 Polyclonal Antibody (14-738) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
3rd Image Caption
Immunofluorescence analysis of U-2 OS cells using RPL32 Polyclonal Antibody (14-738) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 50 uL
Lieferbar: In stock
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