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TUBB8 Antibody

ArtNr PRS-14-544-50uL
Hersteller ProSci
Menge 50 uL
Kategorie
Typ Antibody Primary
Format Liquid
Applikationen WB, IF, IHC
Specific against Human, Mouse, Rat
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias Tubulin beta-8 chain, TUBB8
Lieferbar
Shipping
blue ice or RT
By Research Area
Cell Cycle, Signal Transduction
Immunogen
A synthetic peptide of human TUBB8
Applications
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Positive Control 1
LO2
Positive Control 2
Jurkat
Positive Control 3
HepG2
Positive Control 4
A-549
Positive Control 5
Mouse liver
Positive Control 6
Rat brain
Predicted Molecular Weight
Observed: 54kDa
Purification
Affinity purification
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions
Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
TUBB8
Ncbi Gene Id #
347688
User Note
Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol
TUBB8
Ncbi Official Full Name
Tubulin beta-8 chain
Ncbi Organism
Homo sapiens
Swissprot #
Q3ZCM7
Background
The protein encoded by this gene represents the primary beta-tubulin subunit of oocytes and the early embryo. Defects in this gene, which is primate-specific, are a cause of oocyte maturation defect 2 and infertility.
1st Image Caption
Western blot analysis of extracts of various cell lines, using TUBB8 antibody (14-544) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
2nd Image Caption
Immunohistochemistry of paraffin-embedded human stomach using TUBB8 antibody (14-544) at dilution of 1:100 (40x lens).
3rd Image Caption
Immunohistochemistry of paraffin-embedded rat kidney using TUBB8 antibody (14-544) at dilution of 1:100 (40x lens).
4th Image Caption
Immunofluorescence analysis of U2OS cells using TUBB8 antibody (14-544) at dilution of 1:100. Blue: DAPI for nuclear staining.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 50 uL
Lieferbar: In stock
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