ArtNr |
PRS-14-544-50uL |
Hersteller |
ProSci
|
Menge |
50 uL |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Liquid |
Applikationen |
WB, IF, IHC |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Tubulin beta-8 chain, TUBB8 |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Cell Cycle, Signal Transduction |
Immunogen |
A synthetic peptide of human TUBB8 |
Applications |
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200 |
Positive Control 1 |
LO2 |
Positive Control 2 |
Jurkat |
Positive Control 3 |
HepG2 |
Positive Control 4 |
A-549 |
Positive Control 5 |
Mouse liver |
Positive Control 6 |
Rat brain |
Predicted Molecular Weight |
Observed: 54kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
TUBB8 |
Ncbi Gene Id # |
347688 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
TUBB8 |
Ncbi Official Full Name |
Tubulin beta-8 chain |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q3ZCM7 |
Background |
The protein encoded by this gene represents the primary beta-tubulin subunit of oocytes and the early embryo. Defects in this gene, which is primate-specific, are a cause of oocyte maturation defect 2 and infertility. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using TUBB8 antibody (14-544) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s. |
2nd Image Caption |
Immunohistochemistry of paraffin-embedded human stomach using TUBB8 antibody (14-544) at dilution of 1:100 (40x lens). |
3rd Image Caption |
Immunohistochemistry of paraffin-embedded rat kidney using TUBB8 antibody (14-544) at dilution of 1:100 (40x lens). |
4th Image Caption |
Immunofluorescence analysis of U2OS cells using TUBB8 antibody (14-544) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.