ArtNr |
PRS-14-002-50uL |
Hersteller |
ProSci
|
Menge |
50 uL |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Liquid |
Applikationen |
WB, IF |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
IL1B, interleukin 1, beta, IL-1, IL1-BETA, IL1F2, IL-1 beta, catabolin, interleukin-1 beta, preinterleukin 1 beta, pro-interleukin-1-beta |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Apoptosis, Cell Cycle, Chemokines & Cytokines, Growth Factors, Immunology, Innate Immunity, Neuroscience, Obesity |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 124-223 of human IL1B (NP_000567.1). |
Applications |
WB: 1:500 - 1:2000
IF: 1:50 - 1:200 |
Positive Control 1 |
THP-1 |
Predicted Molecular Weight |
Observed: 37kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
IL1B |
Ncbi Gene Id # |
3553 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
IL1B |
Ncbi Official Full Name |
interleukin 1, beta |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P01584 |
Background |
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using IL1B antibody (14-002) at 1:1000 dilution. THP-1 cells were treated by PMA (80 nM) for overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s. |
2nd Image Caption |
Immunofluorescence analysis of HeLa cells using IL1B antibody (14-002) at dilution of 1:100. Blue: DAPI for nuclear staining. |
3rd Image Caption |
Immunofluorescence analysis of THP-1 cells using IL1B antibody (14-002) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.