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EGFR Antibody

EGFR Antibody

ArtNr	PRS-13-995-50uL
Hersteller	ProSci
Menge	50 uL
Kategorie	Oncology Neuroscience Neural Signaling Protein Aggregation, Clearance & Misfolding Antibodies By Cell Type Blastoma Carcinoma Glioma Hematology Leukemia Lymphoma Myeloma Melanoma Sarcoma Cell Biology Cell Culture Cytokines Adenocarcinoma Immunohistochemistry Immunoprecipitation Western Blot Primary Antibodies Astrocytoma By Organ Bladder Cancer Heart & Blood Cancer
Typ	Antibody Primary
Format	Liquid
Applikationen	WB, IP, IHC
Specific against	Human, Mouse
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR, ERBB, ERBB1, HER1
Lieferbar
Shipping	blue ice or RT
By Research Area	Cancer, Cell Cycle, Growth Factors, Immunology, Signal Transduction
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1021-1210 of human EGFR (NP_005219.2).
Applications	WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IP: 1:50 - 1:100
Positive Control 1	HeLa
Positive Control 2	Mouse liver
Predicted Molecular Weight	Observed: 170kDa
Purification	Affinity purification
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions	Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	EGFR
Ncbi Gene Id #	1956
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	EGFR
Ncbi Official Full Name	epidermal growth factor receptor
Ncbi Organism	Homo sapiens
Swissprot #	P00533
Background	The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.
1st Image Caption	Western blot analysis of extracts of various cell lines, using EGFR antibody (13-995) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
2nd Image Caption	Immunohistochemistry of paraffin-embedded human tonsil using EGFR antibody (13-995) at dilution of 1:150 (40x lens).
3rd Image Caption	Immunohistochemistry of paraffin-embedded human gastric cancer using EGFR antibody (13-995) at dilution of 1:150 (40x lens).
4th Image Caption	Immunohistochemistry of paraffin-embedded mouse testis using EGFR antibody (13-995) at dilution of 1:150 (40x lens).
5th Image Caption	Immunohistochemistry of paraffin-embedded mouse spinal cord endothelial cells using EGFR antibody (13-995) at dilution of 1:150 (40x lens).
6th Image Caption	Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug EGFR antibody (13-995). Western blot was performed from the immunoprecipitate using EGFR antibody (13-995) at a dilition of 1:1000.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 50 uL

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Lieferung vsl. bis 06.09.2024

Express-Lieferung: 7 Tage sparen

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