ArtNr |
PRS-13-728-50uL |
Hersteller |
ProSci
|
Menge |
50 uL |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Liquid |
Applikationen |
WB, IF |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Legumain, Asparaginyl endopeptidase, Protease, cysteine 1, LGMN, PRSC1 |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Cancer, Cell Cycle, Immunology, Signal Transduction |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 18-323 of human LGMN (NP_001008530.1). |
Applications |
WB: 1:1000 - 1:2000
IF: 1:50 - 1:200 |
Positive Control 1 |
Mouse lung |
Positive Control 2 |
Mouse liver |
Predicted Molecular Weight |
Observed: 54kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
LGMN |
Ncbi Gene Id # |
5641 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
LGMN |
Ncbi Official Full Name |
Legumain |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q99538 |
Background |
This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using LGMN antibody (13-728) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s. |
2nd Image Caption |
Immunofluorescence analysis of C6 cells using LGMN Polyclonal Antibody (13-728) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
3rd Image Caption |
Immunofluorescence analysis of HeLa cells using LGMN Polyclonal Antibody (13-728) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
4th Image Caption |
Immunofluorescence analysis of L929 cells using LGMN Polyclonal Antibody (13-728) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.