ArtNr |
PRS-13-114-50uL |
Hersteller |
ProSci
|
Menge |
50 uL |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Liquid |
Applikationen |
WB, IF, IHC |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
PCNA, MGC8367, |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Cancer, Cell Cycle, Signal Transduction, Stem Cell |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human PCNA (NP_002583.1). |
Applications |
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200 |
Positive Control 1 |
A549 |
Positive Control 2 |
MCF7 |
Positive Control 3 |
Jurkat |
Positive Control 4 |
HeLa |
Positive Control 5 |
HepG2 |
Positive Control 6 |
Mouse brain |
Predicted Molecular Weight |
Observed: 36kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
PCNA |
Ncbi Gene Id # |
5111 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
PCNA |
Ncbi Official Full Name |
proliferating cell nuclear antigen |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P12004 |
Background |
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using PCNA antibody (13-114) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s. |
2nd Image Caption |
Immunohistochemistry of paraffin-embedded rat liver using PCNA antibody (13-114) at dilution of 1:100 (40x lens). |
3rd Image Caption |
Immunohistochemistry of paraffin-embedded rat spleen using PCNA antibody (13-114) at dilution of 1:100 (40x lens). |
4th Image Caption |
Immunohistochemistry of paraffin-embedded human stomach using PCNA antibody (13-114) at dilution of 1:100 (40x lens). |
5th Image Caption |
Immunohistochemistry of paraffin-embedded mouse testis using PCNA antibody (13-114) at dilution of 1:100 (40x lens). |
6th Image Caption |
Immunofluorescence analysis of HeLa cells using PCNA antibody (13-114) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.