ArtNr |
NKM-ATGA0598-100ul |
Hersteller |
NKMAX
|
Menge |
100ul |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol |
Applikationen |
ELISA |
Clon |
AT6E6 |
Specific against |
other |
Host |
Mouse |
Isotype |
IgG1 kappa |
Konjugat/Tag |
Unconjugated |
Citations |
Characterization of novel monoclonal antibodies against MERS-coronavirus spike protein{https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114870/} |
NCBI |
AFS88936 |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Middle East respiratory syndrome coronavirus, Human betacoronavirus 2c EMC/2012, MERS-CoV, MERS, MERSCoV SP, Spike glycoprotein, S glycoprotein, S, Spike protein |
Lieferbar |
|
Ab Purification Comment |
By protein-A affinity chromatography |
Ab Usage |
The antibody has been tested by ELISA analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. |
Additional Info Comment |
ELISA: MERS Spike Antibody (1ug/ml) specifically recognizes MERS Spike and Spike S1 recombinant protein, but not interacted MERS Spike S2 and RBD recombinant protein in ELISA. |
Antigen Species |
MERS-CoV |
Clonality Mono VS Poly |
Monoclonal |
Concentration |
1mg/ml (determined by BCA assay) |
Description |
MERS-CoV, which causes the Middles East Respiratory Syndrome (MERS), belongs to a family of viruses known as coronaviruses. MERS-CoV was first identified in the Kingdom of Saudi Arabia in 2012, which is a single and positive stranded RNA virus. Dromedary camels are widely considered as the source of the transmission of MERS-CoV. The rate of human transmission among household contacts of MERS patients has been approximately 5 % based on serological analysis. MERS-CoV has four structural proteins, known as the S (spike), E (envelope), M (membrane), and N (nucleocapsid) proteins. The spike protein, responsible for allowing the virus to attach to and fuse with the membrane of a host cell and is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. MERS-CoV S mediates viral attachment and fusion to human cells via human cellular receptor DPP4, also known as CD26. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. |
Gene Category |
Virus |
General References |
Junghyun Goo., et al. (2020) Virus Res. 278:197863. ; ; Yan-Hua Li., et al. (2019) Engineering. 5:940-947.; ; Lingshu Wang., et al. (2018) J Virol. 92:e02002-2017. ; ; Nicolas Papageorgiou., et al. (2016) Acta Crystallogr D Struct Biol. 72:192-202.; ; Xiao-Yan Che., et al. (2004) J Clin Microbiol. 42:2629-2635.; ; |
Immunogen |
Recombinant MERS-CoV Spike (18-1296aa) purified from Baculovirus |
Product Type Name |
Monoclonal antibody |
Storage |
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
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