Anti-Mouse VEGF - Biotin

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Goat

Immunogen

Purified Recombinant Mouse VEGF (Accession # AAA40547)

Formulation

This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.

Storage and Handling

The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRID

AB_2832133

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Mouse VEGF this biotin conjugate can be used at a concentration of 0.1 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Mouse VEGF is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 µg/ml when used in conjunction with as the capture antibody at 0.2-0.8 µg/ml.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Goat Anti-Mouse Vascular Endothelial Cell Growth Factor (VEGF) recognizes Mouse VEGF. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background

Vascular endothelial growth factor (VEGF), a potent proangiogenic cytokine¹ is the key signal used by oxygen-hungry cells to promote growth of blood vessels. It binds to specialized receptors on the surfaces of endothelial cells and directs them to build new vessels.² VEGF are crucial regulators of vascular development during embryogenesis (vasculogenesis) and blood-vessel formation in the adult (angiogenesis). Abnormal VEGF function is associated with inflammatory diseases including atherosclerosis, and hyperthyroidism.^{3, 4, 5, 6}

Antigen Details

Protein

VEGF

PubMed

VEGF

NCBI Gene Bank ID

22339

UniProt.org

More Information on Uniprot.org

References & Citations

1. Folkman, J. et al. (2008) FASEB J. 22:3728

2. Goodsell, DS. et al. (2002) The Oncologist 7:569

3. Mugishima, H. et al. (2006) J Atheroscler Thromb. 13:130

4. Claesson-Welsh, L. et al. (2006) Exp Cell Res. 312:549

5. Claesson-Welsh, L. et al. (1999) Trends Biochem Sci. 28:488

6. Ellis, LM. et al. (2005) J Clin Oncol. 23:1011

Technical Protocols