Anti-Mouse CD120b (TNFR2) - Purified In vivo PLATINUM™ Functional Grade

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Armenian Hamster

Immunogen

Purified Recombinant Mouse TNF R2

Product Concentration

≥ 5.0 mg/ml

Endotoxin Level

< 0.5 EU/mg as determined by the LAL method

Purity

≥98% monomer by analytical SEC

> 95% by SDS Page

Formulation

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Product Preparation

Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Pathogen Testing

To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.

Storage and Handling

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Country of Origin

USA

Shipping

Ambient

RRID

AB_2832125

Applications and Recommended Usage?
Quality Tested by Leinco

FC The suggested concentration for clone TR75-54.7 antibody for staining cells in flow cytometry is ≤ .25 μg per 10⁶ cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.

Other Applications Reported In Literature ?

IP
B
WB
ELISA Cap

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Anti-Mouse CD120 recognizes Mouse CD120. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution

Variety of cell types at low levels

Background

Tumor necrosis factor receptor II (TNF-RII) is present on most cell types¹ and is considered to play a prominent role in stimulation by TNF-alpha. TNFRII proteins are expressed by hematopoietic cells including macrophages, neutrophils, lymphocytes, thymocytes and mast cells. It is expressed by a variety of other cell types including endothelial cells, cardiac myocytes and prostate cells.²

Antigen Details

Protein

TNFR2

Ligand/Receptor

TNF-α, LT-α (TNF-β)

Function

Apoptosis, NF-κB activation, inflammation, tumor necrosis, cell differentiation

PubMed

TNFR2

NCBI Gene Bank ID

21938

UniProt.org

Information on Uniprot.org

Immunology

.

Innate Immunity

References & Citations

1. Zuckerman, KS. et al. (1998) Cancer Res. 58:2217.
2. Sheehan, KC. et al. (1995) J. Exp. Med. 181:607.

Technical Protocols