Anti-Mouse Vgamma 3 (TCR) - PE

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Syrian Hamster

Immunogen

AKR mouse dendritic epidermal cell clone 7-17

Product Concentration

0.2 mg/ml

Formulation

This R-phycoerythrin (R-PE) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Storage and Handling

This R-PE conjugate is stable when stored at 2-8°C. Do not freeze.

Country of Origin

USA

Shipping

Next Day 2-8°C

Excitation Laser

Blue Laser (488 nm) and/or Green Laser (532 nm)/Yellow-Green Laser (561 nm)

RRID

AB_2894345

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Anti-Mouse Vγ 3 TCR antibody recognizes an epitope of the variable region (Vγ 3) gene product. Antibody from clone 536 immunoprecipitates the TCR heterodimer with two subunits of apparent molecular mass of 52 and 42 kDa.

Antigen Distribution

The antibody 536 stained less than 1% of cells in adult thymus (AKR), splenocytes, lymph node, adult CD4-8- thymocytes, and adult CD4-8-, T splenocyte. 92% of day 14 old BALB/C fetal thymus, 86% of C57BL/6 dEC (Thy-1+) and 94% of AKR dEC (Thy-1+) stained positive using this antibody.

Background

The T cell receptor or TCR is a molecule found on the surface of T lymphocytes that is responsible for recognizing antigens bound to major histocompatibility complex (MHC) molecules. It is a heterodimer consisting of an α and β chain in 95% of T cells, while 5% of T cells have TCRs consisting of γ and δ chains. Engagement of the TCR with antigen and MHC results in activation of its T lymphocyte through a series of biochemical events mediated by associated enzymes, co-receptors and specialized accessory molecules.

Antigen Details

Protein

Vγ 3 T-Cell Receptor

PubMed

Vγ 3 T-Cell Receptor

Research Area

Adaptive Immunity

.

Immunology

References & Citations

Havran, et al. (1989) Pro. Natl. Acad. Sci. USA 86:4185