Anti-Mouse Sca-1 - DyLight 550

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Rat

Immunogen

Purified Recombinant Mouse Sca-1 (> 98%)

Product Concentration

0.2 mg/ml

Formulation

This DyLight® 550 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Storage and Handling

This DyLight^® 550 conjugate is stable when stored at 2-8°C. Do not freeze.

Country of Origin

USA

Shipping

Next Day 2-8°C

Excitation Laser

Yellow Laser (563 nm)

RRID

AB_2831808

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Rat Anti-Mouse Sca-1 (Clone 38/42) recognizes an epitope on Mouse Sca-1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution

Ly-6.2 allotypes (AKR, C57BL, C57BR, C57L, C58, DBA/2, PL, SJL, SWR, 129) express high densities of the Ly-6A/E antigen on peripheral lymphocytes. Ly-6.1 allotypes (A, BALB/C, CBA, C3H/He, DBA/1, NZB) express the Ly6A/E antigen in low density on peripheral lymphocytes. Activated T-cells express the antigen in high density in strains of both allotypes.^{1, 2}

Antigen Details

Protein

Sca-1

PubMed

Sca-1

NCBI Gene Bank ID

110454

UniProt.org

Information on Uniprot.org

Research Area

Immunology

.

Stem Cell

References & Citations

1. Spangrude, G. J. et al. (1991) Science 241:58
2. Codias, E. K. et al. (1989) Immunogenetics 29:08
3. Van de Rijn, M. et al. (1989) Proc. Natl. Acad. Sci. USA 86:4634

Technical Protocols