Antibody Details
Product Details
Reactivity Species
Mouse
Host Species
Rat
Immunogen
E. coli-Derived Recombinant Mouse SCF
Endotoxin Level
< 0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
RRID
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Mouse SCF bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse SCF (at 25 ng/ml) is 5-10 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Description
Specificity
Rat Anti-Mouse Stem Cell Factor (SCF) (Clone 40215.11) recognizes an epitope on Mouse SCF. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody does not cross-react with rhSCF.
Background
Stem cell factor (SCF), otherwise known as KIT ligand or Steel factor1 is a paracrine regulator of germ cell development.2 SCF has the ability to stimulate the growth of hematopoietic progenitor cells and to generate colony-forming cells (CFC) from highly enriched populations of hematopoietic cells.3 SCF is produced by the Sertoli cells and involved in the local regulation of spermatogenesis4 and liver regeneration.5 SCF in conjunction with interleukin-31 may play a significant role in airway remodeling by promoting the recruitment of bone marrow-derived fibroblast precursors into the lung with the capacity to promote lung myofibroblast differentiation.6
Antigen Details
Protein
SCF
PubMed
SCF
References & Citations
1. Toppari, J. et al. (1999) Endocrin 140: 1492
2. Adamson, JW. et al. (1991) PNAS 88: 7420
3. Hervé, L. et al. (2005) J of Endocrin. 186: 131
4. Ramadori, G. et al. (2009) Lab Invest. 89(5):562-74.
6. Phan, SH. et al. (2009) AM J Pathol. 174: 390
Technical Protocols