Anti-Mouse SCF (Clone 40215)

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Rat

Immunogen

E. coli-Derived Recombinant Mouse SCF

Endotoxin Level

< 0.1 EU/µg as determined by the LAL method

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRID

AB_2831790

Other Applications Reported In Literature ?

Neutralization: This antibody is useful for neutralization of Mouse SCF bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Mouse SCF (at 25 ng/ml) is 5-10 µg/ml.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Rat Anti-Mouse Stem Cell Factor (SCF) (Clone 40215.11) recognizes an epitope on Mouse SCF. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody does not cross-react with rhSCF.

Background

Stem cell factor (SCF), otherwise known as KIT ligand or Steel factor¹ is a paracrine regulator of germ cell development.² SCF has the ability to stimulate the growth of hematopoietic progenitor cells and to generate colony-forming cells (CFC) from highly enriched populations of hematopoietic cells.³ SCF is produced by the Sertoli cells and involved in the local regulation of spermatogenesis⁴ and liver regeneration.⁵ SCF in conjunction with interleukin-31 may play a significant role in airway remodeling by promoting the recruitment of bone marrow-derived fibroblast precursors into the lung with the capacity to promote lung myofibroblast differentiation.⁶

Antigen Details

Protein

SCF

PubMed

SCF

References & Citations

1. Toppari, J. et al. (1999) Endocrin 140: 1492

2. Adamson, JW. et al. (1991) PNAS 88: 7420

3. Hervé, L. et al. (2005) J of Endocrin. 186: 131

4. Ramadori, G. et al. (2009) Lab Invest. 89(5):562-74.

6. Phan, SH. et al. (2009) AM J Pathol. 174: 390

Technical Protocols