Anti-Mouse RANKL (CD254) - Biotin

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Rat

Immunogen

Purified Recombinant Mouse RANKL (> 98%)

Product Concentration

0.5 mg/ml

Formulation

This biotinylated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Storage and Handling

This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.

Country of Origin

USA

Shipping

Next Day 2-8°C

RRID

AB_2831712

Applications and Recommended Usage?
Quality Tested by Leinco

FC The suggested concentration for RANKL antibody, clone IK22/5 for staining cells in flow cytometry is ≤ 1.0 μg per 10⁶ cells in a volume of 100 μl or 100 μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Clone IK22/5 recognizes an epitope on mouse RANKL.

Antigen Distribution

RANKL is expressed on activated T cells and osteoclasts.

Background

RANKL antibody, clone IK22/5, recognizes receptor activator of NF-κB ligand (RANKL), also known as CD254, TNF-related activation-induced cytokine (TRANCE), tumor necrosis factor ligand superfamily member 11 (TNFSF11), osteoprotegerin ligand (OPGL), and osteoclast differentiation factor (ODF). RANKL is a type II transmembrane protein belonging to the TNF cytokine superfamily and binds to the signaling receptor, RANK, or decoy receptor, osteoprotegerin (OPG). RANKL is expressed on osteoclasts and is essential for osteoclast differentiation, activation, and survival¹. RANKL is also expressed on activated T cells and regulates T cell-dendritic cell (DC) interactions, enhancing DC activation and survival^2-3. In addition, RANKL is crucial for lymph node, bone marrow, and thymus development⁴. Treatment with denosumab, an antibody directed against RANKL, is approved for the treatment of patients at high risk for bone fractures, including osteoporosis, and has shown anti-tumor effects^5.

Antigen Details

Protein

RANKL

Ligand/Receptor

RANK, OPG

PubMed

RANKL

NCBI Gene Bank ID

21943

UniProt.org

Information on Uniprot.org

Research Area

Cell Biology

.

Immunology

References & Citations

1. Park JH, et al. (2017) Mol Cells. 40(10):706-713

2. Wong BR, et al. (1997) J Biol Chem. 272(40):25190-4

3. Anderson, D. M., et al. (1997) Nature 390:175.

4. Ono T, et al. (2020) Inflamm Regen. 2020; 40:2.

5. de Groot AF, at al. (2018) Cancer Treat Rev. 62:18-28

Technical Protocols