Goat F(ab')2 Anti-Rabbit IgG (H&L) (Adsorbed Against: Hu.) - DyLight 650

Antibody Details

Product Details

Reactivity Species

Rabbit

Host Species

Goat

Immunogen

Purified Rabbit IgG (H& L) (> 98%)

Product Concentration

0.2 mg/ml

Formulation

This DyLight® 650 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Product Preparation

This F(ab')₂ secondary antibody was generated by optimal pepsin degestion of the antisera followed by antigen affinity chromatography to elimiate the presence of Fc fragment and whole molecules.

Storage and Handling

This secondary antibody conjugate should be stored desiccated at 2-8°C.

Expiration Date

One year from date of receipt

Country of Origin

USA

Shipping

Next Day 2-8°C

Working Concentration

It is recommended that working dilutions be prepared fresh daily. FC: 1:100 - 1:800IF: 1:100 - 1:800Secondary antibody concentrations reconstituted with glycerol will be diluted by 50%. Therefore, the working dilution may be one half of what is recommended.

Excitation Laser

Red Laser (652 nm)

RRID

AB_2894225

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Goat F(ab')₂ Anti-Rabbit IgG (H& L) recognizes Rabbit IgG (H& L). This secondary antibody was purified using antigen affinity chromatography and has been adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.

Antigen Details

Protein

IgG (H& L)

PubMed

IgG (H& L)