Anti-Human Raptor (NT)

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Rabbit

Immunogen

PN:R1237

Product Concentration

0.5 mg/ml

Formulation

This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.

Storage and Handling

This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Country of Origin

USA

Shipping

Next Day Ambient

RRID

AB_2831682

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Rabbit Anti-Human Raptor recognizes an epitope near the N-terminus of Human and Mouse Raptor. This polyclonal antibody was purified using affinity chromatography.

Background

The mammalian Target of Rapamycin (TOR, also known as mTOR) is an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors (reviewed in 1). Rapamycin inhibits TOR activity resulting in reduced cell growth and reduced rates of cell cycle and cell proliferation (reviewed in 2). Raptor (regulatory associated protein of TOR) is a TOR-binding protein essential for TOR signaling in vivo. It acts as a TOR scaffold protein whose binding by TOR substrates is necessary for effective TOR-catalyzed phosphorylation.³ These substrates include the ribosomal protein S6 kinase (RP S6K) and the eukaryotic initiation factor 4E binding protein 4EBP1, proteins necessary for cell growth and proliferation and responsive to nutrient and mitogen levels.⁴ Raptor binds these proteins through a common 5 amino acid TOR-signaling (TOS) motif; mutation of this motif prevents the TOR-dependent phosphorylation of these proteins.

Antigen Details

Protein

Raptor

PubMed

Raptor

References & Citations

1. Shamji, AF. et al. (2003) Mol. Cell 12:271
2. Fingar, DC. et al. (2004) Oncogene 23:3151
3. Yonezawa, K. et al. (2004) Biophys. Res. Commun. 313:437
4. Hara, K. et al. (1998) J. Biol. Chem. 273:14484

Technical Protocols