Antibody Details
Product Details
Reactivity Species
Rat
Host Species
Goat
Immunogen
Purified Rat IgG (H& L) (> 98%) containing all isotypes
Product Concentration
1.0 mg/ml
Formulation
This secondary antibody is supplied in 0.05M Tris pH 8.0, containing .001M MgCI2, .001M ZnCI2, 1.0% BSA and 0.09% sodium azide as a preservative.
Product Preparation
This F(ab')2 secondary antibody was generated by optimal pepsin degestion of the antisera followed by antigen affinity chromatography to elimiate the presence of Fc fragment and whole molecules.
Storage and Handling
This secondary antibody conjugate should be stored for up to 4 weeks at 2-8°C. However, for long term storage, aliquot into working volumes without dilution and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day Ambient
Working Concentration
The suggested working dilution for immunohistogy is approximately 1:150. However, each investigator should determine their own optimal working dilution for each specific research application.
RRID
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Description
Specificity
Goat F(ab')2 Anti-Rat IgG (H& L) recognizes Rat IgG (H& L). This secondary antibody was purified using antigen affinity chromatography and has been adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.
Antigen Details
Protein
IgG (H& L)
PubMed
IgG (H& L)