Donkey F(ab’)2 Anti-Rat IgG (H&L) (Adsorbed Against: Ms., Hu., Rb., Bv., Shp., Hs., GP., CK., Sy. Hms.) - AMCA

Antibody Details

Product Details

Reactivity Species

Rat

Host Species

Donkey

Product Concentration

1.5 mg/ml

Formulation

This secondary antibody is lyophilized from 0.01M phosphate buffered saline (PBS) pH 7.6 containing 0.25M NaCl, 15 mg/ml IgG free BSA and 0.05% sodium azide as a preservative.

Product Preparation

This F(ab')₂ secondary antibody was generated by optimal pepsin degestion of the antisera followed by antigen affinity chromatography to elimiate the presence of Fc fragment and whole molecules.

Storage and Handling

This lyophilized secondary antibody conjugate should be stored desiccated at 2-8°C. The reconstituted AMCA conjugate containing glycerol should be stored at -20°C in a manual defrost freezer.

Expiration Date

One year from date of reconstitution

Country of Origin

USA

Shipping

Next Day Ambient

Working Concentration

1:50 - 1:200 for most applications.

RRID

AB_2894198

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Donkey F(ab')₂ Anti-Rat IgG (H& L) recognizes Rat IgG heavy and light chains. This secondary antibody was purified using antigen affinity chromatography and adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.

Antigen Details

Protein

IgG (H& L)

PubMed

IgG (H& L)