Goat Anti-Rabbit IgG (H&L) (Adsorbed Against: Hu., Bv.) - HRP

Antibody Details

Product Details

Reactivity Species

Rabbit

Host Species

Goat

Immunogen

Purified Rabbit IgG (H& L) (> 98%)

Product Concentration

0.5 mg/ml

Formulation

This HRP-conjugated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4, 1% BSA. (Warning: Use of sodium azide as a preservative will inhibit the enzyme activity of horseradish peroxidase)

Storage and Handling

This HRPO conjugate is stable when stored at 2-8°C. Do not freeze.

Country of Origin

USA

Shipping

Next Day 2-8°C

Working Concentration

1:500 - 1:5, 000 for immunohistochemistry on tissue sections
1:5, 000 - 1:100, 000 for ELISA and Western blotting with chromogenic substrates
1:10, 000 - 1:200, 000 for Western blotting with ECL substrates

RRID

AB_2810875

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Goat Anti-Rabbit IgG (H& L) recognizes Rabbit IgG (H& L). This secondary antibody was purified using antigen affinity chromatography and adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.

Antigen Details

Protein

IgG (H& L)

PubMed

IgG (H& L)

References & Citations

1.) Mackay, J. P. et al. (2020) Cell. 33: 9, 108450 Journal Link

2.) Mesa RA and Roberson EDO. (2020) F1000Research. 8:1211 Journal Link