Goat F(ab’)2 Anti-Rabbit IgG (H&L) (Adsorbed Against: Hu., Bv.) - Texas Red

Antibody Details

Product Details

Reactivity Species

Rabbit

Host Species

Goat

Immunogen

Purified Rabbit IgG (H& L) (> 98%)

Product Concentration

1.0 mg/ml

Formulation

This secondary antibody is formulated inin 0.01M phosphate buffered saline (PBS) pH 7.4, containing 1.0% BSA, 1.0% glycerol and 0.09% sodium azide as a preservative. The specific antibody concentration of this conjugate is 1.0 mg per ml.

Product Preparation

This F(ab')₂ secondary antibody was generated by optimal pepsin degestion of the antisera followed by antigen affinity chromatography to elimiate the presence of Fc fragment and whole molecules.

Storage and Handling

Stable when stored at 2 - 8°C. Do not freeze.

Country of Origin

USA

Shipping

Next Day 2-8°C

Working Concentration

The suggested working dilution for immunohistology is approximately 1:100. However, each investigator should determine their own optimal working dilution for each specific research application.

RRID

AB_2894165

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Goat F(ab')₂ Anti-Rabbit IgG (H& L) recognizes Rabbit IgG (H& L). This secondary antibody was purified using antigen affinity chromatography and has been adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.

Antigen Details

Protein

IgG (H& L)

PubMed

IgG (H& L)

References & Citations

1. Fujiki, Y. et al. (2002) J Biol Chem. 277(46):44513-24. Article Link

2. Fujiki, Y. et al. (2000) J Biol Chem. 275(28):21703-14. Article Link

3. Fujiki, Y. et al. (2000) J Biol Chem. 275(28):21715-21. Article Link

Technical Protocols