Vergleich

Anti-Mouse NK1.1 Antibody - Purified in vivo GOLD™ Functional Grade

ArtNr LEIN-N123-100mg
Hersteller Leinco Technologies
Menge 100 mg
Quantity options 1 mg 100 mg 25 mg 5 mg 50 mg
Kategorie
Typ Antibody Monoclonal
Applikationen WB, FC, IP, other, IV
Clon PK136
Specific against Mouse (Murine, Mus musculus)
Host Mouse
Purity > 95% by SDS-PAGE and ≥ 95% monomer by analytical SEC
Citations 1.) Ardolino, M. et al. (2018) J Clin Invest. 128(10):4654-4668.
2.) Koo, GC. and Peppard, JR. (1986) Hybridoma 3:301
3.) Raju et al. (2019) Cell Reports. 29:2556–2564
NCBI 17059
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Applications
Quality Tested by Leinco Flow Cytometry: The suggested use of this antibody is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Manufacturer - Category
Purified in vivo Functional Grade>Gold Purified Bulk Antibodies|Purified in vivo Functional Grade|Primary Monoclonal Antibodies>Tumor Markers
Manufacturer - Targets
NK1.1
Country of Origin
USA
Shipping Temperature
Next Day 2-8°C
Storage Conditions
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Product Description
NK1.1 is a type II membrane protein that is part of the C-type lectin superfamily. NK1.1 contains a transmembrane domain and a cytoplasmic domain that are characteristic of C-type lectins. The function of NK cells is to mediate cytotoxicity and to secrete cytokines after immune stimulation. NK1.1 has been correlated with lysis of tumor cells In vitro and rejection of bone marrow allografts In vivo. It is also involved in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 is commonly used as a mouse NK cell marker.
Background
NK1.1 is a type II membrane protein that is part of the C-type lectin superfamily. NK1.1 contains a transmembrane domain and a cytoplasmic domain that are characteristic of C-type lectins. The function of NK cells is to mediate cytotoxicity and to secrete cytokines after immune stimulation. NK1.1 has been correlated with lysis of tumor cells In vitro and rejection of bone marrow allografts In vivo. It is also involved in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 is commonly used as a mouse NK cell marker.
Function
NK cell activation, IFN-γ production, cytotoxic granule release
Other Applications Reported In Literature
CyTOF® (Validated)
IP
Depletion
Block
IHC
IF (Reported in the literature)
PubMed
NK1.1
Manufacturer - Research Area
Immunology, Innate Immunity
Manufacturer - Specificity
Clone PK136 recognizes mouse NK1.1.
RRID
AB_2737553
Concentration
≥ 5.0 mg/ml
Formulation
This monoclonal NK1.1 antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Antigen Distribution
NK-1.1 is encoded by the NKR-P1B/NKR-P1C gene and expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129.
Endotoxin Level
≤ 1.0 EU/mg
Immunogen
NK-1+ cells from mouse spleen and bone marrow
Additonal Information
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 mg
Lieferbar: In stock
lieferbar

Lieferung vsl. bis 31.01.2025 

Vergleichen

Auf den Wunschzettel

Angebot anfordern

Lieferzeit anfragen

Technische Frage stellen

Bulk-Anfrage stellen

Fragen zum Produkt?
 
Schließen