Anti-Mouse ICAM-1

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Goat

Immunogen

NS0-Derived Recombinant Mouse ICAM-1 Extracellular Domain

Endotoxin Level

< 0.1 EU/µg as determined by the LAL method

Formulation

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

Storage and Handling

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRID

AB_2830862

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Mouse ICAM-1 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Mouse ICAM-1 is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.

Other Applications Reported In Literature ?

Blocking: This antibody shows inhibition in the HSB2 adhesionassay. The adhesion of PMA treated human HSB2 cells (5 x 104 cells/well) to12.5 μg/mL immobilized rmICAM-1/Fc (100 μL/well) was maximally inhibited(80 - 100%) following pre-treatment with antibody for 1 hour using 100 μL of a5 μg/mL antibody solution per well.
Immunohistochemistry: Suitable for use at concentration of 5-15 µg/mL.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Goat Anti-Mouse Intercellular Adhesion Molecule-1 (ICAM-1) recognizes Mouse ICAM-1. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Antigen Distribution

CD54 is present on endothelial cells, lymphocytes, epithelial cells, dendritic cells and keratinocytes.

Background

ICAM-1 is a 55 kDa glycoprotein that is part of the Ig superfamily. It is heavily glycosylated to form 75 kDa to 115 kDa. ICAM-1 is known to be an adhesion and viral entry molecule, and its long suspected involevement in signal transduction is being elucidated. The signal-transducing functions of ICAM-1 appear to be mainly associated with proinflammatory pathways. Furthermore, ICAM-1 signaling appears to act as a beacon for inflammatory immune cells such as macrophages and granulocytes bringing about inflammation via lymphocyte trafficking. ICAM-1 is essential for the transmigration of leukocytes out of blood vessels and into tissues, and is a marker of endothelial dysfunction leading to damaging vascular disorders in umbilical and placental vascular tissue of gestational pregnancies. ICAM-1 is the receptor for rhinoviruses (the cause of most common colds) and malaria, and plays an inflammatory role in ocular allergies.

Antigen Details

Protein

ICAM-1

PubMed

ICAM-1

NCBI Gene Bank ID

15894

UniProt.org

More Information on Uniprot.org

References & Citations

1. Li, S. et al. (2009) Biochem Biophys Res Commun. 381: 459

2. Wolf, S. et al. (2009) Pharmacol Rep 61: 22

3. Ozcan, U et al. (2009) Arch Gynecol Obstet.

Technical Protocols