Anti-Rat IL-1beta (Clone 38139)

Antibody Details

Product Details

Reactivity Species

Rat

Host Species

Mouse

Immunogen

E. coli-Derived Recombinant Rat Interleukin-1β

Endotoxin Level

< 0.1 EU/µg as determined by the LAL method

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRID

AB_2830793

Applications and Recommended Usage?
Quality Tested by Leinco

Indirect ELISA: This antibody can be used in an indirect detection ELISA at 2-8 µg/mL with a suitable second step reagent such as PN:M114. The detection sensitivity of this indirect ELISA for Rat IL-1β is approximately 25 ng/well.
Western Blotting: To detect Rat IL-1β this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for IL-1β is approximately 100 ng/lane and 50 ng/lane under non-reducing and reducing conditions, respectively. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Clone 38139 recognizes an epitope on rat IL-1β. tibody has less than 5% cross-reactivity with rhIL-1α, rmIL-1α and rrIL-1α.

Background

IL-1R1 is an 80 kD transmembrane glycoprotein and a member of the Ig superfamily. IL-1 binds both IL-1R1 and IL-1R2. The ligands IL-1α and IL-1β only commence when binding IL-1R1 because IL-1R2 acts as a decoy receptor and competitively antagonizes the binding of IL-1α and IL-1β, thereby reducing their biological effects. Binding of the ligands to IL-1R1 is enhanced by an accessory protein, IL-1R-AcP. IL-1α can exist as either a 31 kD precursor or an 18 kD mature form. IL-1α is a proinflammatory cell-associated cytokine and IL-1β is a 17.5 kD proinflammatory secreted cytokine. Both IL-1α and IL-1β bind to the same receptor and have similar properties that include stimulation of thymocyte proliferation via IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. Furthermore, IL-1β mediates a variety of immune and inflammatory responses. IL-1β is a major cause of severe inflammation in the mouse model of Crohn’s Disease, which makes IL-1β an important target for this disease.

Antigen Details

Protein

IL-1β

PubMed

IL-1β

References & Citations

1. Dinarello, CA. et al. (1996) Blood 87: 2095

2. Yuan, J. et al. (1993) Cell 75: 641

3. Maeda, S. et al. (2005) Science 4: 735

Technical Protocols