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Anti-Human IL-1 RI (Clone 35730)

ArtNr LEIN-I-642-25ug
Hersteller Leinco Technologies
Menge 25ug
Kategorie
Typ Antibody Monoclonal
Applikationen WB, ELISA
Clon 35730
Specific against other
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Lieferbar
info

Antibody Details

Product Details

Reactivity Species

Human


Host Species

Mouse


Immunogen

Purified Recombinant Human IL-1 RI (> 98%)

Endotoxin Level

< 0.1 EU/µg as determined by the LAL method


Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA


Shipping

Next Day Ambient

RRID

AB_2830764

Applications and Recommended Usage?
Quality Tested by Leinco

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:I-766 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:I-556.
Western Blotting: To detect Human IL-1 RI this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human IL-1 RI is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-556.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Human Interleukin 1 Receptor Type I (IL-1 RI) (Clone 35730) recognizes an epitope on Human IL-1 RI. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. No cross-reactivity was observed with rhIL-1 RII, rhIL-1 R5 or rmIL-1 RI.

Antigen Details

Protein

IL-1 RI

PubMed

IL-1 RI

Technical Protocols



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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 25ug
Lieferbar: In stock
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