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Anti-Human CD195 (CCR5) - Purified in vivo GOLD™ Functional Grade

Anti-Human CD195 (CCR5) - Purified in vivo GOLD™ Functional Grade

ArtNr	LEIN-I-2501-1.0mg
Hersteller	Leinco Technologies
Menge	1 mg
Quantity options	1 mg 100 mg 25 mg 5 mg 50 mg
Kategorie	In Vivo Antibodies In Vivo Antibodies
Typ	Antibody Monoclonal
Applikationen	FC, ICC, IV
Clon	HEK/1/85a/7a
Specific against	Human (Homo sapiens)
Host	Rat
Purity	> 95% by SDS-PAGE and ≥ 95% monomer by analytical SEC
Citations	1. Mackay CR., et al. (1997) Proc Natl Acad Sci. 94(5):1925-30 2. Doms RW., et al. (1999) Proc Natl Acad Sci. 96(9):5215-5220 3. Parmentier M., et al. (1996) Biochemistry. 35(11):3362-7 4. Berger EA., et al. (1996) Science. 272(5270):1955-8 5. Sodroski J., et al. (1996) Cell. 1996 85(7):1135-48 6. Doms RW., et al. (1996) Cell. 85(7):1149-58 7. Paxton WA., et al. (1996) Nature. 381(6584):667-73 8. Sheppard HW., et al. (1997) Nat Med. 3(10):1160-2 9. Delovitch TL., et al. (2000) J Immunol. 165(2):1102-10 10. Casagrande N., et al. (2020) Cancers (Basel). 12(7):1765
NCBI	1234
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Applications	Quality Tested by Leinco FC The suggested concentration for this CD195 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 10⁶ cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Manufacturer - Category	Purified in vivo Functional Grade>Gold Purified Bulk Antibodies\|Primary Monoclonal Antibodies>Growth Factors and Cytokines\|Purified in vivo Functional Grade\|Primary Monoclonal Antibodies>Surfacetag Mouse CD Markers
Manufacturer - Targets	CD195
Country of Origin	USA
Shipping Temperature	Next Day 2-8°C
Storage Conditions	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Product Description	CCR5 antibody, HEK/1/85a/7a, recognizes C-C chemokine receptor type 5 (CCR5), also known as CD195, CC-CKR-5, CHEMR13, and HIV-1 fusion coreceptor. CCR5 is a 45 kDa seven-transmembrane G-protein-coupled receptor (GPCR). CCR5 is expressed on a subset of memory T cells, macrophages, and immature dendritic cells (DCs) and is upregulated by proinflammatory cytokines1, 2. CCR5 binds to inflammatory CC-chemokines, including CCL3 (macrophage inflammatory protein (MIP)-1α), CCL4 (MIP-1β), and RANTES, which increases intracellular calcium levels and results in cytoskeletal rearrangement and chemotactic cell migration3. CCR5 is also the coreceptor for HIV-1 following binding of viral gp120 to CD44-7. The levels of CCR5 on cells influence the rate of entry of HIV-1, and patients with lower levels of CCR5 exhibit immunity against infection8. CCR5 also contributes to other human diseases, including type I diabetes9 and cancer10.
Background	CCR5 antibody, HEK/1/85a/7a, recognizes C-C chemokine receptor type 5 (CCR5), also known as CD195, CC-CKR-5, CHEMR13, and HIV-1 fusion coreceptor. CCR5 is a 45 kDa seven-transmembrane G-protein-coupled receptor (GPCR). CCR5 is expressed on a subset of memory T cells, macrophages, and immature dendritic cells (DCs) and is upregulated by proinflammatory cytokines^{1, 2}. CCR5 binds to inflammatory CC-chemokines, including CCL3 (macrophage inflammatory protein (MIP)-1α), CCL4 (MIP-1β), and RANTES, which increases intracellular calcium levels and results in cytoskeletal rearrangement and chemotactic cell migration³. CCR5 is also the coreceptor for HIV-1 following binding of viral gp120 to CD4^4-7. The levels of CCR5 on cells influence the rate of entry of HIV-1, and patients with lower levels of CCR5 exhibit immunity against infection⁸. CCR5 also contributes to other human diseases, including type I diabetes⁹ and cancer¹⁰.
Other Applications Reported In Literature	ICC
PubMed	CD195
Manufacturer - Specificity	Clone HEK/1/85a/7a recognizes an epitope on human CCR5.
RRID	AB_2893835
Concentration	≥ 5.0 mg/ml
Formulation	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Antigen Distribution	CCR5 is expressed on memory T cells, macrophages, and immature dendritic cells (DCs).
Endotoxin Level	≤ 1.0 EU/mg
Immunogen	CHO cells transfected with full-length human CD195 (CCR5).
Additonal Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

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LEIN-I-2501-1.0mg-safety-datasheet.pdf