Antibody Details
Product Details
Reactivity Species
Human
Host Species
Goat
Immunogen
Purified Recombinant IP-10 (> 98%)
Endotoxin Level
< 0.1 EU/µg as determined by the LAL method
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
RRID
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA: This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human CXCL10/IP‑10/CRG‑2 Monoclonal Antibody.
Flow Cytometry: Suitable for use at concentration of 2.5 µg/106 cells.
Other Applications Reported In Literature ?
Immunohistochemistry: Suitable for use at concentration of 1-15 µg/mL.
CyTOF-ready:Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry: Suitable for use at concentration of 5-15 µg/mL.
Neutralization:Measured by its ability to neutralize CXCL10/IP‑10/CRG‑2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR3. The Neutralization Dose (ND50) is typically 1-4 µg/mL in the presence of 0.2 µg/mL Recombinant Human CXCL10/IP‑10/CRG‑2.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Description
Specificity
Goat Anti-Human Chemokine (C-X-C Motif) Ligand 10 (CXCL10) recognizes Human CXCL10. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
Chemokine (C-X-C motif) ligand 10 (CXCL10) or IP-10 is a small cytokine belonging to the CXC chemokine family. CXCL10 is secreted by several cell types in response to IFN-γ. These cell types include monocytes, endothelial cells and fibroblasts.1 CXCL10 has been attributed to several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis.2, 3 CXCL10 plays a role in the regulation of tubular cell proliferation following renal ischemia-reperfusion injury.4
Antigen Details
Protein
CXCL10
PubMed
CXCL10
NCBI Gene Bank ID
3627
UniProt.org
More Information on Uniprot.org
References & Citations
1. Luster, AD. et al.(1985) Nature 315: 672
2. Dufour, JH. et al.(2002) J. Immunol. 168
3. Angiolillo, AL. et al.(1995) J. Exp. Med. 182: 155
4. Kaneko, S. et al.(2008) Nephron Exp. Nephrol. 109:(1):c29-38
Technical Protocols