Anti-Human IgG Fc Specific - HRP

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Mouse

Product Concentration

0.5 mg/ml

Formulation

This HRP-conjugated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4, 1% BSA. (Warning: Use of sodium azide as a preservative will inhibit the enzyme activity of horseradish peroxidase)

Product Preparation

Clone HP6043, Anti-Human IgG Fc Specific monoclonal antibody has been purified using multi-step affinity chromatography methods using protein A.

Storage and Handling

This conjugated antibody is stable when stored at 2-8°C. Do not freeze.

Cross Reactivity

Cross-reactivity by ELISA against human myeloma proteins:
Human IgG1: 100%
Human IgG2: 100%
Human IgG3: 100%
Human IgG4: 100%
Human IgM: < 0.2%
Human IgA: < 0.1%
Human IgE: < 0.1%

There is no detectable binding to bovine, goat, horse sheep IgG.

NOTE: This Anti-Human IgG, Fc Fragment Specific (Clone HP6043) when conjugated to HRPO is validated for use in Enzyme Immunoassay for the detection of Human IgG, Fc Fragment Specific (Part No.: I-1200)

Country of Origin

USA

Shipping

Next Day Ambient

RRID

AB_2893814

Applications and Recommended Usage?
Quality Tested by Leinco

Enzyme Immunoassay 1:1000; see application references

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

This antibody reacts with all IgG isotypes and is non-reactive with IgA, IgM, IgE, light chains or F(ab')₂ portions of human IgG.

Ligand/Receptor

CD16, CD32, CD64

References & Citations

1. Reimer, C. B. et al. (1984) Hybridoma 3:263
2. Jefferies, R. et al. (1985) Immunol. Letters 10:223
3. Phillips, D. J. et al. (1987) Immunol. Letters 17:159

Technical Protocols