Anti-Human/Mouse Integrin beta7 - DyLight 650

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Rat

Immunogen

TK1 Cells

Product Concentration

0.2 mg/ml

Formulation

This DyLight® 650 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Storage and Handling

This DyLight® 650 conjugate is stable when stored at 2-8°C. Do not freeze.

Country of Origin

USA

Shipping

Next Day 2-8°C

Excitation Laser

Red Laser (652 nm)

RRID

AB_2830490

Applications and Recommended Usage?
Quality Tested by Leinco

Flow Cytometry: The suggested concentration of use for clone FIB504 antibody for staining cells in flow cytometry is ≤0.25 µg per 10⁶ cells in a volume of 100 μl or 100 μl of whole blood. Each lot of this antibody is quality control tested.

Other Applications Reported In Literature ?

Additional reported applications (for the relevant formats) include:

B This antibody has been reported to block beta7 integrin mediated cell adhesion for In vitro and In vivo studies.

Western Blotting

Immunoprecipitation

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Clone FIB504 recognizes an epitope on human/mouse integrin β7.

Antigen Distribution

Integrin α4β7 is expressed on subsets of peripheral lymphocytes, thymocytes, and bone marrow progenitors. The αEβ7 integrin is expressed on mucosal T cells, including intraepithelial T lymphocytes (IELs) and lamina propria T cells, subsets of dendritic cells, and regulatory T cells (Tregs)⁸.

Background

Integrin β7 antibody, clone FIB504, recognizes integrin β7, a 130 kDa membrane glycoprotein of the Ig superfamily. Integrin β7 forms heterodimers with both the α4 (CD49d) and aE (CD103) integrins. α4β7 integrin is expressed on subsets of peripheral lymphocytes, thymocytes, and bone marrow progenitors^{1, 2}. It mediates adhesion to mucosal endothelial cells, promoting leukocyte transendothelial migration through interactions with mucosal addressin cell adhesion molecule-1 (MAdCAM-1)^{3, 4}. The αEβ7 integrin is expressed on mucosal T cells, including intraepithelial T lymphocytes (IELs) and lamina propria T cells^{5, 6}, subsets of dendritic cells⁷, and regulatory T cells (Tregs)8. It facilitates retention in the gut epithelial layer via interactions with E-cadherin⁹. Anti-β7 antibodies block both the homing to and retention in the gut of pathogenic T cells¹⁰ and are currently under evaluation in a phase 3 clinical trial to treat inflammatory bowel disease¹¹.

Antigen Details

Protein

Integrin Beta 7

Ligand/Receptor

CD49d/β7: VCAM-1 (CD106), MAdCAM-1 and fibronectin; CD103/β7: E-cadherin

PubMed

Integrin Beta 7

NCBI Gene Bank ID

16421

UniProt.org

Information on Uniprot.org

Research Area

Cell Biology

.

Immunology

.

Neuroscience

References & Citations

1. Andrew DP, et al. (1996) Eur J Immunol. 26(4):897-905

2. Murakami JL, et al. (2016) Stem Cells Dev. 25(1):18-26

3. Hu MC, et al. (1992) Proc Natl Acad Sci USA. 89(17):8254-8

4. Hamann A, et al. (1994) J Immunol. 152(7):3282-93

5. Hadley GA Higgins JM. (2014) Adv Exp Med Biol. 819:97–110

6. Farstad IN., et al. (1996) Immunology. 89:227–37

7. Jaensson E., et al. (2008) J Exp Med. 205:2139–49

8. Allez M, et al. (2002) Gastroenterology. 123(5):1516-26

9. Schön MP, et al. (1999) J Immunol. 162(11):6641-9

10. Stefanich EG, et al. (2011) Br J Pharmacol. 162(8):1855-1870

11. Smids C, et al. (2017) J Crohns Colitis. 11(4):500-508

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