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Anti-Mouse IL-4 (Clone 11B11) - Purified in vivo GOLD™ Functional Grade

Anti-Mouse IL-4 (Clone 11B11) - Purified in vivo GOLD™ Functional Grade

ArtNr	LEIN-I-1071-5mg
Hersteller	Leinco Technologies
Menge	5 mg
Quantity options	100 mg 1 mg 1 mg 25 mg 50 mg 5 mg
Kategorie	In Vivo Antibodies In Vivo Antibodies
Typ	Antibody Monoclonal
Applikationen	IP, IHC, ICC, ELISA, FA, IV
Clon	11B11
Specific against	Mouse (Murine, Mus musculus)
Host	Rat
Purity	> 95% by SDS-PAGE and ≥ 95% monomer by analytical SEC
Citations	1. Yoshimoto T & Paul WE. (1994) J Exp Med. 179(4):1285–95 2. Kipnis J., at al. (2012) J Immunol. 189(9):4213-4219 3. Groth B., et al. (1992) J Exp Med. 176:1091–8 4. Murphy KM., et al. (1992) Proc Natl Acad Sci USA. 89:6065–9 5. Paul WE., et al. (1999) Annual Rev Immunol. 17:701-38 6. Maquart FX., et al. (2000) Lab Invest. 80(8):1337-43 7. Godeau, G., et al. (2000) Lab Invest. 80,1337–1343 8. Steinke JW & Borish L. (2001) Respir Res. 2(2):66-70
NCBI	16189
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Applications	Quality Tested by Leinco ELISAThis antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 0.5-2.0 μg/ml. Titration of the reagent is recommended for optimal performance for each application.
Manufacturer - Category	Purified in vivo Functional Grade>Gold Purified Bulk Antibodies\|Primary Monoclonal Antibodies>Surfacetag Mouse CD Markers
Manufacturer - Targets	IL-4
Country of Origin	USA
Shipping Temperature	Next Day 2-8°C
Storage Conditions	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Product Description	IL-4 antibody, 11B11, recognizes mouse IL-4, also known as B-cell differentiation factor (BCDF) and B-cell stimulatory factor (BSF1). IL-4 is a pleiotropic 20 kDa cytokine secreted by T helper 2 cells (Th2) cells, natural killer T (NKT) cells, mast cells, eosinophils, and basophils1, 2. IL-4 signaling through the IL-4 receptor alpha chain (IL-4Ra) induces the differentiation of naive T cells to Th2 cells3, 4 and stimulates B cell differentiation, proliferation, and class switching to IgG1 and IgE isotypes5. In addition, IL-4 is implicated in normal wound healing6, 7 and airway inflammation in patients with asthma8.
Background	IL-4 antibody, 11B11, recognizes mouse IL-4, also known as B-cell differentiation factor (BCDF) and B-cell stimulatory factor (BSF1). IL-4 is a pleiotropic 20 kDa cytokine secreted by T helper 2 cells (Th2) cells, natural killer T (NKT) cells, mast cells, eosinophils, and basophils^{1, 2}. IL-4 signaling through the IL-4 receptor alpha chain (IL-4Ra) induces the differentiation of naive T cells to Th2 cells^{3, 4} and stimulates B cell differentiation, proliferation, and class switching to IgG1 and IgE isotypes⁵. In addition, IL-4 is implicated in normal wound healing^{6, 7} and airway inflammation in patients with asthma⁸.
Other Applications Reported In Literature	N IHC ICC IP CyTOF® FA
PubMed	IL-4
Manufacturer - Specificity	Clone 11B11 recognizes an epitope on mouse IL-4.
RRID	AB_2830405
Concentration	≥ 5.0 mg/ml
Formulation	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Antigen Distribution	IL-4 is secreted by Th2 cells, NKT cells, mast cells, eosinophils, and basophils.
Endotoxin Level	≤ 1.0 EU/mg
Immunogen	Partially Purified Native Mouse IL-4
Additonal Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

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LEIN-I-1071-5mg-safety-datasheet.pdf