Anti-Human HLA-DQ (MHC Class II)- APC

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Mouse

Product Concentration

0.2 mg/ml

Formulation

This Allophycocyanin (APC) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Storage and Handling

This APC conjugate is stable when stored at 2-8°C. Do not freeze.

Country of Origin

USA

Shipping

Next Day 2-8°C

Excitation Laser

Red Laser (650 nm)

RRID

AB_2893760

Applications and Recommended Usage?
Quality Tested by Leinco

FC

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Clone 1a3 recognizes a monomorphic epitope on human HLA-DQ1. It does not cross-react with HLA-DR or HLA-DP.

Antigen Distribution

HLA-DQ is expressed on antigen-presenting cells, including macrophages, monocytes, DCs, and B cells, and activated T cells.

Background

HLA-DQ antibody, clone 1a3, recognizes the major histocompatibility complex (MHC) class II molecule Human Leukocyte Antigen - DQ isotype (HLA-DQ). MHC class II is constitutively expressed on human professional antigen-presenting cells (APCs), including macrophages/monocytes, dendritic cells (DCs), and B cells, and is induced on T cells upon activation². HLA-DQ consists of two transmembrane proteins, a 35 kDa α (heavy) chain and 29 kDa β (light) chain³ encoded by the HLA-DQA1 and HLA-DQB1 genes, respectively, located in the HLA complex of chromosome 6. The N-terminal α1 and β1 domains form the antigen-binding groove, which binds 13-25 aa peptides derived from exogenous antigens⁴. On APCs, MHC class II plays a critical role in the adaptive immune response by presenting phagocytosed antigens to helper CD4 T cells. The T cell receptor (TCR)/CD3 complex of CD4 T cells interacts with peptide-MHC class II, which induces CD4 T cell activation leading to the coordination and regulation of other effector cells. CD4 molecules also bind to MHC class II, which helps augment TCR signaling⁵. It has also been demonstrated that MHC class II express on activated T cells are capable of antigen presentation⁶ and can transduce signals into T cells, enhancing T cell proliferation and activity⁷. Specific alleles of HLA-DQ are associated with autoimmune diseases, including celiac disease⁸ and type 1 diabetes⁹, and graft-versus-host disease¹⁰.

Antigen Details

Protein

HLA-DQ Monomorphic

Ligand/Receptor

CD3/TCR, CD8

PubMed

HLA-DQ Monomorphic

NCBI Gene Bank ID

3122

Research Area

Immunology

.

Innate Immunity

References & Citations

1. Shookster L, et al. (1987) Hum Immunol. 20(1):59-70

2. Holling TM, Schooten E, van Den Elsen PJ. (2004) Hum Immunol. 65(4):282-90

3. Mitaksov V, Fremont DH. (2006) J Biol Chem. 281(15):10618-25

4. Wieczorek M, et al. (2017) Front Immunol. 8:292

5. Artyomov MN, et al. (2010) Proc Natl Acad Sci USA. 107(39):16916-16921

6. Barnaba V, et al (1994) Eur J Immunol. 24(1):71-5

7. Di Rosa F, et al. (1993) Hum Immunol. 38(4):251-60

8. Castaño L, et al. (2004) J Pediatr Gastroenterol Nutr. 39:80–84

9. Cucca F, et al. (1993) Hum Immunol. 37:85 –94

10. Petersdorf EW, (1996) Proc Natl Acad Sci USA. 93(26):15358-63

Technical Protocols