Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Goat

Immunogen

Sf 21 - Derived Recombinant Mouse HGF Receptor Extracellular Domain

Endotoxin Level

< 0.1 EU/µg as determined by the LAL method

Formulation

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

Storage and Handling

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRID

AB_2830323

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Mouse HGF R this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Mouse HGF R is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.

Other Applications Reported In Literature ?

IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 2 - 15 µg/ml. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
Immunocytochemistry: Suitable for use at concentration of 5-15 µg/mL.
Blocking: In a functional ELISA, 0.3-1 µg/mL of this antibody will block 50% of the binding of 5 ng/mL of Recombinant Human HGF to immobilized Recombinant Mouse HGF R/c-MET Fc Chimera coated at 1 µg/mL (100 µL/well). At 20 μg/mL, this antibody will block > 90% of the binding.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Goat Anti-Mouse Hepatocyte Growth Factor Receptor (HGF R) recognizes Mouse HGF R. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background

Hepatocyte growth factor receptor (HGFR), also known as c-Met, plays an important roles in angiogenesis and tumor growth.¹ C-Met is a receptor tyrosine kinase expressed by epithelial cells of the brain, kidney, liver and other tissues. Binding of its ligand, Hepatocyte Growth Factor (HGF), triggers receptor autophosphorylation, and activation of several downstream effectors including the mitogen-activated protein kinases ERK-1 and ERK-2, and PLC-γ. Activation of the c-Met signal transduction pathway leads to mulitple cellular responses including cell motility, scattering, proliferation, survival and angiogenesis.^{2, 3}

Antigen Details

Protein

HGF R

PubMed

HGF R

NCBI Gene Bank ID

17295

UniProt.org

More Information on Uniprot.org

References & Citations

1. McDonald, DM. et al. (2008) BMB Rep. 41: 833

2. Park, M. et al. (1998) Oncogene 16: 833

3. Comoglio, PM. et al. (1998) J Cell Sci. 111: 237

Technical Protocols