Anti-Human GITR

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Goat

Immunogen

NS0-Derived Recombinant Human GITR Extracellular Domain

Endotoxin Level

< 0.1 EU/µg as determined by the LAL method

Formulation

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

Storage and Handling

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRID

AB_2830274

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Human GITR this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for rhGITR isapproximately 1 ng/lane and 25 ng/lane under non-reducing and reducing conditions, respectively. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Flow Cytometry: Suitable for use at concentration of2.5 µg/10⁶ cells.

Other Applications Reported In Literature ?

IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 10 µg/ml. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
Blocking: Approximately 1 - 2 μg/mL of this antibody will block 50% of the binding of 10 ng/mL of rhGITR Ligand to immobilized rhGITR/Fc chimera (100 μL of a 2 μg/mL solution was coated in each well) in a functional ELISA.
CyTOF-ready:Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Goat Anti-Human Glucocorticoid Induced TNF Receptor (GITR) recognizes Human GITR. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background

GITR (glucocorticoid-induced tumor necrosis factor receptor) also known as TNFRSF18, is a surface receptor molecule that has been shown to be involved in inhibiting the suppressive activity of T-regulatory cells and extending the survival of T-effector cells.¹ GITR is involved in the regulation of T-cell receptor mediated cell death. Constitutive expression of a transfected GITR gene induces resistance to apoptosis induced by anti CD3 monoclonal antibodies. GITR ligand interferes with the suppressor activity of CD4(+) CD25(+) regulatory T-cells.²

Antigen Details

Protein

GITR

PubMed

GITR

NCBI Gene Bank ID

8784

UniProt.org

More Information on Uniprot.org

References & Citations

1. Shimizu, J. et al. (2002) Nature Immunology 3:135–142.

2. Ji, Hb. et al. (2004)Journal of Immunology 172: 5823

Technical Protocols