Anti-Influenza A, Nucleocapsid (Clone 1078)

Antibody Details

Product Details

Reactivity Species

Influenza A Virus

Expression Host

HEK-293

Immunogen

Sequenced from human patients

Product Concentration

≥ 5.0 mg/ml

Endotoxin Level

≤ 1.0 EU/mg as determined by the LAL method

Purity

≥95% monomer by analytical SEC

Formulation

This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Storage and Handling

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Country of Origin

USA

Shipping

Ships Overnight on Blue Ice

RRID

AB_2893599

Applications and Recommended Usage?
Quality Tested by Leinco

ELISA

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Anti-Influenza A, Nucleocapsid (Clone 1078) specifically targets an epitope on the Influenza A Nucleocapsid Protein

Antigen Distribution

The nucleocapsid protein is expressed in the internal nucleocapsid of Influenza A

Background

There are four types of Influenza (flu) viruses: type A, B, C, and D. Influenza A and B viruses are routinely spread in people and other mammals, while also known for seasonal flu epidemics each year. Influenza A is the genus Alphainfluenzavirus of the virus family Orthomyxoviridae. Influenza A is classified into two main protein subtypes, hemagglutinin (HA) and neuraminidase (NA), located on the surface of the virus. Breaking down the proteins further, there are 18 hemagglutinin subtypes and 11 different neuraminidase subtypes¹. Influenza A can be even further classified into specific clades (also known as groups) and sub-clades (also known as sub-groups) based on similarity of their HA/NA gene sequences¹. The Nucleocapsid protein or nucleoprotein (NP) of the influenza virus A negative-strand RNA’s primary function is to encapsulate the virus genome for the purpose of RNA transcription, replication and packaging². Specifically, NP is the most abundant viral protein in infected cells², therefore the NP can be and has been used for anti-influenza drug development³ The NP of influenza A and B viruses share up to 38% of their amino acid sequence, indicating region functionality differences at the amino acid level⁴

Antigen Details

Protein

Influenza A Nucleocapsid

PubMed

Influenza A Nucleocapsid

Research Area

Infectious Disease

References & Citations

1. Types of Influenza viruses (2019) Center for Disease Control and Prevention.
2. Hu Y, Sneyd H, et al. (2017) Curr Top med Chem. 2017; 17(20): 2271–2285.
3. Portela A, Digard P. (2012) J. Gen Virol. 2002 Apr; 83(Pt 4):723-734.
4. Sherry L, Smith M, et al. (2014) Journal of Virology. 2014; 88(21); 12326-12338. DOI https://doi.org/10.1128/JVI.01542-14

Technical Protocols