Anti-Human Eotaxin-3

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Goat

Immunogen

E. coli-Derived Recombinant Human Eotaxin-3/CCL26 (aa 24 - 94)

Endotoxin Level

< 0.1 EU/µg as determined by the LAL method

Formulation

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

Storage and Handling

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRID

AB_2829988

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Human Eotaxin-3 this polyclonal antibody can be used at a concentration of 1 - 2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human Eotaxin-3 is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.

Other Applications Reported In Literature ?

Immunohistochemistry on Frozen Sections: This antibody should give satisfactory staining results when used on frozen sections at a concentration of 15 µg/ml.
Neutralization: This antibody is useful for neutralization of Human Eotaxin-3 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human Eotaxin-3 (at 1 ng/ml) is 10 - 25 µg/ml.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Goat Anti-Human Eotaxin-3 recognizes Human Eotaxin-3. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background

Eotaxin-3/CCL-26 belongs to a family of CC chemokines, which are potent chemoattractants for eosinophils that signal through the CCR3 receptor. ¹ It is produced by endothelial cells stimulated with IL-4 or IL-13. Eotaxin-3 selectively targets cells expressing CCR3, including eosinophils, basophils, T cells and monocytes. It has been reported that eotaxin-3 is an antagonist for CCR2.² Eotaxin-3 has similar activity to Eotaxin and Eotaxin-2, but the three Eotaxins share only a low degree of sequence homology. Eotaxin-3 is up-regulated in the context of a T helper 2 mediated inflammatory bowel disease via the signal transducer and activator of transcription 6, thus suggesting that the intestinal epithelium actively participates in the recruitment of eosinophils at the site of inflammation.¹

Antigen Details

Protein

Eotaxin-3

PubMed

Eotaxin-3

NCBI Gene Bank ID

56221

UniProt.org

More Information on Uniprot.org

References & Citations

1. Cuber, JC.et al. (2005) Int J Biochem Cell biol. 37: 2559

2. Gerber, B. et al. (2004) J Biol Chem. 279: 23357

Technical Protocols