AffiPure Normal Donkey IgG, Whole Molecule

Antibody Details

Product Details

Host Species

Donkey

Product Concentration

5.0 - 15 mg/ml

Formulation

This product has been aseptically packaged and formulated in phosphate buffered saline (PBS) pH 7.1-7.5 with no carrier proteins or preservatives.
Product may be provided with carrier proteins or preservatives by request

State of Matter

Sterile Liquid

Storage and Handling

Store at 2-8° C under sterile conditions

Expiration Date

One year after date of receipt

Country of Origin

USA

Shipping

Next Day 2-8°C

RRID

AB_2893594

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Background

AffiPure™ Normal Donkey IgG, Whole Molecule was purified using protein G chromatography from normal serum. IgG is produced and secreted by plasma B cells, and constitutes the majority of serum immunoglobulins. The whole IgG molecule contains both the F(c) region, recognized by high-affinity Fc receptor proteins, and the F(ab) region with the epitope-recognition site, and thus contains two antigen binding sites. IgG antibodies mediate immunological responses to allergy and pathogenic infections by binding to viruses, bacteria, or fungi and facilitating their destruction by activation of the compliment cascade, immobilizing them via agglutination, or opsinization for phagocytosis. This product contains a sufficient quantity of endogenous IgG to block nonspecific binding sites on fixed samples and assay substrates. While most commonly used in flow cytometry this product is useful in other applications such as ELISA, IHC, ICC, Blocking and as a control.

References & Citations

1. Tsuji, H. et al. (2001) Biol Pharm Bull. 24(11):1235-40. Article Link