Anti-Human CD16 (Clone 3G8) - Purified In vivo PLATINUM™ Functional Grade

Antibody Details

Product Details

Reactivity Species

Baboon

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Chimpanzee

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Cynomolgus Monkey

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Marmoset

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Pigtailed Macaque

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Rhesus Monkey

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Squirrel Monkey

Human

Host Species

Mouse

Immunogen

Human PMN cells

Product Concentration

≥ 5.0 mg/ml

Endotoxin Level

< 0.5 EU/mg as determined by the LAL method

Purity

≥98% monomer by analytical SEC

> 95% by SDS Page

Formulation

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Product Preparation

Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Pathogen Testing

To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM^TM antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.

Storage and Handling

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Country of Origin

USA

Shipping

Next Day 2-8°C

RRID

AB_2829822

Applications and Recommended Usage
Quality Tested by Leinco

FC The suggested concentration for this 3G8 antibody for staining cells in flow cytometry is ≤ 2.0 μg per 10⁶ cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.

Other Applications Reported In Literature

CyTOF®
IHC (Frozen)
IP
B

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Clone 3G8 recognizes an epitope on human CD16. This clone also cross-reacts with non-human primate CD16.

Antigen Distribution

CD16 is expressed on NK cells, activated monocytes, macrophages, neutrophils, and placental trophoblasts.

Background

CD16 antibody, 3G8, recognizes human CD16, also known as low-affinity IgG receptor III (FcγRIII). There are two distinct forms of CD16, CD16a (FcγRIIIa) and CD16b (FcγRIIIb). CD16a is a 50-65 kDa heterooligomeric polypeptide-anchored transmembrane protein expressed by NK cells, macrophages, and subsets of monocytes¹. CD16b is a 48 kDa monomeric glycosylphosphatidylinositol (GPI)-anchored protein expressed on neutrophils¹. Upon binding to the Fc portion of IgG or IgG-antigen complex, both CD16 isoforms induce signaling cascades resulting in multiple functions, including phagocytosis, cytokine release, proliferation, degranulation, and antibody dependent cell-mediated cytotoxicity (ADCC)².

Antigen Details

Protein

CD16

Ligand/Receptor

Aggregated IgG, IgG-antigen complex

Function

Low affinity IgG Fc receptor, phagocytosis, ADCC

PubMed

CD16

NCBI Gene Bank ID

2214

UniProt.org

Information on Uniprot.org

Immunology

.

Innate Immunity

References & Citations

1. Ravetch JV & Perussia B. (1989) J Exp Med. 170(2):481-497

2. Nimmerjahn F & Ravetch JV (2008) Nat Rev Immunol. 8(1):34-47

Technical Protocols