Anti-Human CD51 (Clone 13C2) - Purified

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Mouse

Immunogen

Human Osteoclastoma Tissue

Product Concentration

0.1 mg/ml

Formulation

This purified monoclonal antibody is formulated in phosphate buffered saline (PBS), 1 mg/ml BSA and 10 mM sodium azide as a preservative.

Storage and Handling

For use within 1 month of purchase store at 2-8°C. For long term storage freeze working aliquots at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Country of Origin

USA

Shipping

Next Day Ambient

RRID

AB_2829792

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Human CD51 (Clone 13C2) recognizes Human CD51. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. The antibody recognizes a 110/90 kDa antigen known as the alpha-chain of the vitronectin receptor (VNR). This forms a complex with the CD61 antigen and mediates cell adhesion to RGD (arg-gly-asp) containing sequences in vitronectin, von Willebrand factor, fibrinogen and thrombospondin. This antibody reacts with osteoclasts, placenta and melanoma cell lines, some endothelial cells, smooth muscle cells and fibroblasts, and weakly with platelets.

Antigen Details

Protein

CD51

Ligand/Receptor

Vitronectin, von Willebrand factor, fibrinogen, thrombospondin, osteopontin, denatured collagen, fibronectin, laminin.

PubMed

CD51

NCBI Gene Bank ID

3685

UniProt.org

Information on Uniprot.org

References & Citations

1. Strobel, I. et al. (2000) Gene Therapy 7:2028
2. Leukocyte Typing IV (1989) Oxford University Press
3. Davies, J. et al. (1989) J. Cell Biol. 109:1817
4. Horton, M. A. et al. (1985) Cancer Research 45: 5663

Technical Protocols