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Anti-Mouse CD155 (PVR) - Purified In vivo PLATINUM™ Functional Grade

ArtNr LEIN-C6020-5.0mg
Hersteller Leinco Technologies
Menge 5 mg
Quantity options 100 mg 1 mg 25 mg 5 mg 50 mg
Kategorie
Typ Antibody Primary
Applikationen FC, IV
Clon 4.24.1
Specific against other
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Lieferbar
info

Antibody Details

Product Details

Reactivity Species

Mouse


Host Species

Rat


Immunogen

EL4 transfected with mouse CD155

Product Concentration

≥ 5.0 mg/ml


Endotoxin Level

< 0.5 EU/mg as determined by the LAL method


Purity

≥98% monomer by analytical SEC

> 95% by SDS Page


Formulation

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Product Preparation

Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Pathogen Testing

To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.

Storage and Handling

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Country of Origin

USA


Shipping

Next Day 2-8°C

RRID

AB_2829775

Applications and Recommended Usage
Quality Tested by Leinco

FC The suggested concentration for this 4.24.1 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Clone 4.24.1 recognizes an epitope on mouse CD155.

Antigen Distribution

CD155 is expressed at cell junctions on the primary vascular endothelial cells and is highly expressed on DP thymocytes.

Background

CD155 is a transmembrane glycoprotein member of a subfamily of immunoglobulin-like adhesion receptors (nectins). CD155 is commonly known as Poliovirus Receptor (PVR) because of its involvement as a cellular receptor for poliovirus. The normal function of CD155 is to establish intercellular adherens junctions between epithelial cells. Of its 3 extracellular immunoglobulin-like domains (D1, D2, and D3), the virus only recognizes D1. CD155 interacts with both CD226 and CD96 to induce cytotoxicity of NK cells and CTL. The precise role of CD155 in the immune system remains to be elucidated, though it is suspected to be involved in intestinal humoral immune responses. Furthermore, it is thought that CD155 may be used to positively select MHC-independent T cells in the thymus.

Antigen Details

Protein

CD155

Ligand/Receptor

CD226 (DNAM-1), CD96

Function

Apart from its function of adhering junction among contacting epithelial cells, the interaction of DNAM-1 (CD226) with its ligands CD155 and CD112 (nectin 2) induces cytotoxcity of NK cells and CD8+ T cells and cytokine secretion.

PubMed

CD155

NCBI Gene Bank ID

58998

UniProt.org

Information on Uniprot.org

Immunology

.

Innate Immunity


Technical Protocols



Manufacturer - Target
 CD155

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 5 mg
Lieferbar: In stock
lieferbar

Lieferung vsl. bis 13.12.2024 

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