Antibody Details
Product Details
Reactivity Species
Mouse
Host Species
Rat
Immunogen
EL4 transfected with mouse CD155
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 0.5 EU/mg as determined by the LAL method
Purity
≥98% monomer by analytical SEC
> 95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
RRID
Applications and Recommended Usage
Quality Tested by Leinco
FC The suggested concentration for this 4.24.1 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Description
Specificity
Clone 4.24.1 recognizes an epitope on mouse CD155.
Antigen Distribution
CD155 is expressed at cell junctions on the primary vascular endothelial cells and is highly expressed on DP thymocytes.
Background
CD155 is a transmembrane glycoprotein member of a subfamily of immunoglobulin-like adhesion receptors (nectins). CD155 is commonly known as Poliovirus Receptor (PVR) because of its involvement as a cellular receptor for poliovirus. The normal function of CD155 is to establish intercellular adherens junctions between epithelial cells. Of its 3 extracellular immunoglobulin-like domains (D1, D2, and D3), the virus only recognizes D1. CD155 interacts with both CD226 and CD96 to induce cytotoxicity of NK cells and CTL. The precise role of CD155 in the immune system remains to be elucidated, though it is suspected to be involved in intestinal humoral immune responses. Furthermore, it is thought that CD155 may be used to positively select MHC-independent T cells in the thymus.
Antigen Details
Protein
CD155
Ligand/Receptor
CD226 (DNAM-1), CD96
Function
Apart from its function of adhering junction among contacting epithelial cells, the interaction of DNAM-1 (CD226) with its ligands CD155 and CD112 (nectin 2) induces cytotoxcity of NK cells and CD8+ T cells and cytokine secretion.
PubMed
CD155
NCBI Gene Bank ID
58998
UniProt.org
Information on Uniprot.org
Immunology
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Innate Immunity
Technical Protocols