Anti-Mouse ICAM-1 (CD54) (Clone BE29G1) - PE

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Rat

Immunogen

Purified Recombinant Mouse ICAM-1 (> 98%)

Product Concentration

0.2 mg/ml

Formulation

This R-phycoerythrin (R-PE) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Storage and Handling

This R-PE conjugate is stable when stored at 2-8°C. Do not freeze.

Country of Origin

USA

Shipping

Next Day 2-8°C

Excitation Laser

Blue Laser (488 nm) and/or Green Laser (532 nm)/Yellow-Green Laser (561 nm)

RRID

AB_2829568

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Rat Anti-Mouse Inter-Cellular Adhesion Molecule 1 (ICAM-1) (Clone BE29G1) recognizes an epitope on Mouse ICAM-1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution

The CD54 antigen is present on endothelial cells, lymphocytes, epithelial cells, dendritic cells and keratinocytes. It has been demonstrated that ICAM-1 enhances T-cell activation.

Background

Intercellular adhesion molecule-1 (ICAM-1) is one of an important cell adhesion molecules (CAMs) family transmembrane glycoprotein¹ and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines.² ICAM-1 is a marker of endothelial dysfunction leading to damaging vascular disorders, in umbilical and placental vascular tissue of gestational pregnancies.³

Antigen Details

Protein

Inter-Cellular Adhesion Molecule 1

Ligand/Receptor

CD11a/CD18 (LFA-1) or CD11b/CD18 (Mac-1) and CD11c/CD18, CD43, hyaluronan, fibrinogen

PubMed

Inter-Cellular Adhesion Molecule 1

NCBI Gene Bank ID

15894

UniProt.org

Information on Uniprot.org

Research Area

Cell Adhesion

.

Cell Biology

.

Costimulatory Molecules

.

Immunology

.

Innate Immunity

.

Neuroscience

.

Neuroscience Cell Markers

.

Stem Cell

References & Citations

1. Li S et al. (2009) Biochem Biophys Res Commun. 381: 459
2. Wolf S et al. (2009) Pharmacol Rep 61: 22
3. Ozcan U et al. (2009) Arch Gynecol Obstet.

Technical Protocols