Anti-Mouse CD28 - PE

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Syrian Hamster

Immunogen

Purified Recombinant Mouse CD28 (> 98%)

Product Concentration

0.2 mg/ml

Formulation

This R-phycoerythrin (R-PE) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Storage and Handling

This R-PE conjugate is stable when stored at 2-8°C. Do not freeze.

Country of Origin

USA

Shipping

Next Day 2-8°C

Excitation Laser

Blue Laser (488 nm) and/or Green Laser (532 nm)/Yellow-Green Laser (561 nm)

RRID

AB_2829544

Applications and Recommended Usage?
Quality Tested by Leinco

FC The suggested concentration for this 37.51 antibody for staining cells in flow cytometry is ≤ 0.5 μg per 10⁶ cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.

Additional Reported Applications For Relevant Conjugates ?

B
Cosim
IP
IHC (Frozen)
For specific conjugates of this clone, review literature for suggested application details.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Clone 37.51 recognizes an epitope on mouse CD28.

Antigen Distribution

CD28 is expressed on thymocytes, CD4+, CD8+ peripheral T cells, NK cells.

Background

CD28 is a 44 kD glycoprotein and a member of the Ig superfamily. In its capacity as a costimulatory receptor, CD28 produces co-stimulatory signals necessary for T cell activation and survival. CD28 is the only B7 receptor constitutively expressed on naive T cells. Without having this CD28/B7 interaction, T cells will fail to respond to their specific antigen when coming in contact with an MHC/antigen complex and thus, are said to be anergic.

Antigen Details

Protein

CD28

Ligand/Receptor

CD80 (B7-1), CD86 (B7-2)

PubMed

CD28

NCBI Gene Bank ID

12487

UniProt.org

Information on Uniprot.org

Research Area

Costimulatory Molecules

.

Immunology

References & Citations

Gross, J. A. et al. (1992) J. Immunol. 149:380

Technical Protocols