Antibody Details
Product Details
Reactivity Species
Mouse
Host Species
Syrian Hamster
Immunogen
Purified Recombinant Mouse CD28 (> 98%)
Product Concentration
0.2 mg/ml
Formulation
This R-phycoerythrin (R-PE) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This R-PE conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day 2-8°C
Excitation Laser
Blue Laser (488 nm) and/or Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this 37.51 antibody for staining cells in flow cytometry is ≤ 0.5 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Additional Reported Applications For Relevant Conjugates ?
B
Cosim
IP
IHC (Frozen)
For specific conjugates of this clone, review literature for suggested application details.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Description
Specificity
Clone 37.51 recognizes an epitope on mouse CD28.
Antigen Distribution
CD28 is expressed on thymocytes, CD4+, CD8+ peripheral T cells, NK cells.
Background
CD28 is a 44 kD glycoprotein and a member of the Ig superfamily. In its capacity as a costimulatory receptor, CD28 produces co-stimulatory signals necessary for T cell activation and survival. CD28 is the only B7 receptor constitutively expressed on naive T cells. Without having this CD28/B7 interaction, T cells will fail to respond to their specific antigen when coming in contact with an MHC/antigen complex and thus, are said to be anergic.
Antigen Details
Protein
CD28
Ligand/Receptor
CD80 (B7-1), CD86 (B7-2)
PubMed
CD28
NCBI Gene Bank ID
12487
UniProt.org
Information on Uniprot.org
Research Area
Costimulatory Molecules
.
Immunology
References & Citations
Gross, J. A. et al. (1992) J. Immunol. 149:380
Technical Protocols