ArtNr |
LEIN-C2443-5.0mg |
Hersteller |
Leinco Technologies
|
Menge |
5 mg |
Quantity options |
0.5 mg
1 mg
5 mg
|
Kategorie |
|
Typ |
Antibody Monoclonal |
Applikationen |
FC, IHC, IV |
Clon |
17A2 |
Specific against |
Mouse (Murine, Mus musculus) |
Host |
Rat |
Purity |
> 95% by SDS-PAGE and ≥ 95% monomer by analytical SEC |
Citations |
1. Gallimore, A. et al. (2005) J Immunol. 175(11):7098-102. 2. Mysliwietz, J. et al. (1992) Blood 80: 2661-2667 |
NCBI |
12502 |
ECLASS 10.1 |
42030590 |
ECLASS 11.0 |
42030590 |
UNSPSC |
12352203 |
Versandbedingung |
Gekühlt |
Lieferbar |
|
Manufacturer - Applications |
Quality Tested by Leinco FC The suggested concentration for this 17A2 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. |
Country of Origin |
USA |
Shipping Temperature |
2-8°C |
Storage Conditions |
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
Background |
CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition. |
Function |
Antigen recognition, TCR signal transduction, T cell activation |
Other Applications Reported In Literature |
CODEX® IHC (Frozen) The suggested concentration for this 17A2 antibody in IHC staining on frozen tissue is 5.0 - 10 μg per ml. Titration of the reagent is recommended for optimal performance for each application. |
PubMed |
CD3 |
Ligand/Receptor |
Peptide antigen/MHC-complex |
Manufacturer - Specificity |
Clone 17A2 recognizes an epitope on mouse CD3. |
RRID |
AB_2737469 |
Concentration |
≥1.0 mg/ml |
Formulation |
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
Product Preparation |
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
Antigen Distribution |
CD3 is expressed on human peripheral blood lymphocytes, splenic lymphocytes, and the majority of T-CLL and T-ALL cells. |
Endotoxin Level |
≤ 1.0 EU/mg |
Immunogen |
γ/δ TCR-positive T-T hybridoma D1 |
Additonal Information |
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. |