Anti-Mouse CD252 (OX40L) - DyLight 650

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Rat

Immunogen

Rat NRK-52E cell line transfected with mouse OX-40L gene

Product Concentration

0.2 mg/ml

Formulation

This DyLight® 650 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Product Preparation

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Followed by purification this antibody was conjugated to DyLight 650 using optimal conditions.

Storage and Handling

This DyLight^® 650 conjugate is stable when stored at 2-8°C. Do not freeze.

Country of Origin

USA

Shipping

Next Day 2-8°C

Excitation Laser

Red Laser (652 nm)

RRID

AB_2829437

Applications and Recommended Usage?
Quality Tested by Leinco

For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. Each lot of this antibody is quality control tested.

Other Applications Reported In Literature ?

Additional reported applications (for the relevant formats)

B:The RM134L antibody can block the costimulatory activity of OX40L.

IHC-FFPE

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Conjugation Details

Reporter Molecule

DyLight®650 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.

Description

Specificity

Rat Anti-Mouse OX 40 Ligand (CD252) (Clone RM134L) recognizes an epitope on Mouse CD252.

Antigen Distribution

Activated B cells, antigen presenting cells

Background

CD252 is a 35 kD member of the TNF superfamily and is the ligand for OX-40, which is primarily found on activated T cells. The CD252/OX-40 pair contributes to B-cell and T-cell interaction during the immune response. Both proliferative and IL-2 production responses of activated T cells are enhanced via OX-40 antigen stimulation, while proliferation and Ig secretion by activated B cells is enhanced via CD252 stimulation. Hence, because both T cells and B cells are activated in cross-linking, the CD252/OX-40 pair is considered reciprocally costimulatory.

Antigen Details

Protein

OX 40 Ligand

Ligand/Receptor

OX40 (CD134)

Function

B-cell/T-cell interaction, costimulation

PubMed

OX 40 Ligand

NCBI Gene Bank ID

22164

UniProt.org

Information on Uniprot.org

Research Area

Costimulatory Molecules

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Immunology

Technical Protocols