Anti-Mouse CD18 - Purified

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Rat

Product Concentration

0.5 mg/ml

Formulation

This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Storage and Handling

This purified antibody is stable when stored at 2-8°C. Do not freeze.

Country of Origin

USA

Shipping

Next Day Ambient

RRID

AB_2829400

Applications and Recommended Usage?
Quality Tested by Leinco

FC The suggested concentration for this C71/16 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 10⁶ cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.

Other Applications Reported In Literature ?

IHC (Frozen)
IP

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Clone C71/16 recognizes an epitope on mouse CD18.

Antigen Distribution

CD18 is present on mouse T-cells, B-cells, NK cells, monocytes, macrophages and granulocytes.

Background

CD18 is a 95kD type I transmembrane glycoprotein. CD18 is the Integrin β2 chain and forms multiple heterodimers with the three integrin α chains, CD11a, CD11b, and CD11c. CD18 binds with CD11a to form LFA-1. LFA-1 is involved in adhesion and binding to antigen presenting cells via interactions with ICAM-1. CD18 binds with CD11b and CD11c forming complement receptors , such as MAC-1, that participate in the innate immune response by recognizing foreign antigen peptides and destroying them via phagocytosis. In humans, lack of functional CD18 causes Leukocyte Adhesion Deficiency (LAD) which is characterized by the inability of circulating leukocytes to respond to foreign bodies, reducing the ability of the immune system to fight off infection.

Antigen Details

Protein

CD18

Ligand/Receptor

ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), iC3b, fibrinogen

PubMed

CD18

NCBI Gene Bank ID

16414

UniProt.org

Information on Uniprot.org

Research Area

Cell Biology

.

Neuroinflammation

.

Neuroscience

References & Citations

Springer, T. et al. (1978) Eur. J. Immunol. 8:539

Technical Protocols