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Anti-Mouse CD115 (CSF-1R) Antibody - Purified in vivo GOLD™ Functional Grade

Anti-Mouse CD115 (CSF-1R) Antibody - Purified in vivo GOLD™ Functional Grade

ArtNr	LEIN-C2169-25mg
Hersteller	Leinco Technologies
Menge	25 mg
Quantity options	1 mg 100 mg 25 mg 5 mg 50 mg
Kategorie	In Vivo Antibodies In Vivo Antibodies
Typ	Antibody Monoclonal
Applikationen	FC, FA, other, IV
Clon	AFS98
Specific against	Mouse (Murine, Mus musculus)
Host	Rat
Purity	> 95% by SDS-PAGE and ≥ 95% monomer by analytical SEC
Citations	1. Stanley ER., et al. (1982) Cell. 28:71–81 2. Roussel MF., at al. (1988) Cold Spring Harb Symp Quant Biol. 53:521–530 3. Park-Min, KH., et al. (2020) Exp Mol Med 52, 1239–1254 4. Tak, P. P., at al. (2016) Nat. Rev. Drug Disco. 16, 53–70 5. d’Amore F. (2014) Histopathology. 65:490–500 6. Wei YQ., et al. (2012) PLoS One. 7:e50946 7. Rüttinger D., et al. (2017) J Immunother Cancer. 5(1):53
NCBI	12978
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Applications	Quality Tested by Leinco FC The suggested concentration for this AFS98 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 10⁶ cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Manufacturer - Category	Purified in vivo Functional Grade>Gold Purified Bulk Antibodies\|Purified in vivo Functional Grade\|Primary Monoclonal Antibodies>Surfacetag Mouse CD Markers
Manufacturer - Targets	CD115
Country of Origin	USA
Shipping Temperature	Next Day 2-8°C
Storage Conditions	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Product Description	CD115 antibody, clone AFS98, recognizes CD115, also known as mouse colony-stimulating factor 1 receptor (CSF-1R and macrophage colony-stimulating factor receptor (M-CSFR). CD115 is a 150kDa single-pass type I membrane protein encoded by the c-fms gene that belongs to the type III protein tyrosine kinase receptor family. CD115 has an immunoglobulin-like extracellular domain, transmembrane domain, and C-terminal tail receptor and is expressed by monocytes, macrophages, plasmacytoid and conventional dendritic cells (DCs), osteoclasts, and their precursors. CD115 is the receptor for CSF1, also known as M-CSF, and IL-34. Binding and signaling through CD115 regulates the proliferation, differentiation, survival, and cytokine-production of monocytes and macrophages1, 2. In addition, CD115 plays a key role in the differentiation and proliferation of osteoclasts as well as their bone resorption activity3. Mutations in CSF-1R are associated with cancer, including myeloid malignancies, Alzheimer’s disease, and other inflammatory and autoimmune diseases4. The presence of tumor-associated macrophages (TAMs) expressing CSF-1R correlates with poor survival in various tumor types5, 6, and efforts to eliminate these TAMs using CSF1R small-molecule inhibitors and monoclonal antibodies are currently in clinical trials7.
Background	CD115 antibody, clone AFS98, recognizes CD115, also known as mouse colony-stimulating factor 1 receptor (CSF-1R and macrophage colony-stimulating factor receptor (M-CSFR). CD115 is a 150kDa single-pass type I membrane protein encoded by the c-fms gene that belongs to the type III protein tyrosine kinase receptor family. CD115 has an immunoglobulin-like extracellular domain, transmembrane domain, and C-terminal tail receptor and is expressed by monocytes, macrophages, plasmacytoid and conventional dendritic cells (DCs), osteoclasts, and their precursors. CD115 is the receptor for CSF1, also known as M-CSF, and IL-34. Binding and signaling through CD115 regulates the proliferation, differentiation, survival, and cytokine-production of monocytes and macrophages^{1, 2}. In addition, CD115 plays a key role in the differentiation and proliferation of osteoclasts as well as their bone resorption activity³. Mutations in CSF-1R are associated with cancer, including myeloid malignancies, Alzheimer’s disease, and other inflammatory and autoimmune diseases⁴. The presence of tumor-associated macrophages (TAMs) expressing CSF-1R correlates with poor survival in various tumor types^{5, 6}, and efforts to eliminate these TAMs using CSF1R small-molecule inhibitors and monoclonal antibodies are currently in clinical trials⁷.
Other Applications Reported In Literature	B CyTOF® FA Depletion
PubMed	CD115
Ligand/Receptor	Macrophage colony stimulating factor (M-CSF), IL-34
Manufacturer - Specificity	Clone AFS98 recognizes an epitope on mouse CD115.
RRID	AB_2737459
Concentration	≥ 5.0 mg/ml
Formulation	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Antigen Distribution	CD115 (CSF-1R) is expressed on monocytes/macrophages, peritoneal exudate cells, plasmacytoid cells, dendritic cells, and osteoclasts.
Endotoxin Level	≤ 1.0 EU/mg
Immunogen	Not available
Additonal Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

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LEIN-C2169-25mg-safety-datasheet.pdf