Anti-Mouse CD32/CD16 - DyLight 488

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Rat

Product Concentration

0.2 mg/ml

Formulation

This DyLight® 488 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Storage and Handling

This DyLight^® 488 conjugate is stable when stored at 2-8°C. Do not freeze.

Country of Origin

USA

Shipping

Next Day 2-8°C

Excitation Laser

Blue Laser (493 nm)

RRID

AB_2829157

Applications and Recommended Usage?
Quality Tested by Leinco

FC The suggested concentration for this 2.4G2 antibody for staining cells in flow cytometry is ≤ 1 μg per 10⁶ cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.

Additional Reported Applications For Relevant Conjugates ?

B
For specific conjugates of this clone, review literature for suggested application details.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

https://www.thermofisher.com/antibody/product/MA1-10301.html?CID=AFLLO-MA1-10301

Antigen Distribution

These receptors are present on B cells, monocyte/macrophages, NK cells, neutrophils, mast cells and dendritic cells.

Background

CD16 is expressed in two forms: CD16a and CD16b. CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. CD16b (FcγRIIIB) is a 48 kD GPI-anchored protein whose extracellular domain is over 95% homologous to that of CD16a. CD16 regulates both phagocytosis and antibody-dependent cell-mediated cytotoxicity. It has been reported that CD16 is involved in Natural Killer Cell activation and plays a role in signal transduction. The receptors, CD32 (FcγRIII) and CD16 (FcγRII), are 40-60 kD and bind antibody-antigen immune complexes and mediate adaptive immune responses.

Antigen Details

Protein

CD32/CD16

Ligand/Receptor

IgG

PubMed

CD32/CD16

NCBI Gene Bank ID

14130 14131

UniProt.org

Information on Uniprot.org

Research Area

Immunology

.

Innate Immunity

References & Citations

1. Titas, J. A. et al. (1982) J. Immunol. 133:556
2. Rodewald, H. et al. (1992) Cell 69:139

Technical Protocols