Antibody Details
Product Details
Reactivity Species
Human
Host Species
Mouse
Product Concentration
0.1 mg/ml
Formulation
This PerCP conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This PerCP conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day 2-8°C
RRID
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this UCHT-1 antibody for staining cells in flow cytometry is ≤ .25 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Description
Specificity
Clone UCHT-1 recognizes an epitope on human CD3ε.
Antigen Distribution
CD3 is expressed on human peripheral blood lymphocytes, splenic lymphocytes, and the majority of T-CLL and T-ALL cells.
Background
CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.
Antigen Details
Protein
CD3
Ligand/Receptor
Peptide antigen bound to MHC
PubMed
CD3
NCBI Gene Bank ID
915
UniProt.org
Information on Uniprot.org
Research Area
Immunology
References & Citations
1. van Dongen, J. et al. (1988) Blood 71:603
2. Meuer, SC. et al. (1983) Nature 303:808
3. Beverly, P. et al. (1981) Eur. J. Immunol. 11:329
4. Leukocyte Typing III: Code No. 126, 208 and 471; 4th Workshop: Paper No. T3.2
Technical Protocols