Anti-Human Caspase-3 - DyLight 550

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Mouse

Immunogen

Purified Recombinant Human CASP3 (> 98%)

Product Concentration

0.2 mg/ml

Formulation

This DyLight® 550 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Storage and Handling

This DyLight^® 550 conjugate is stable when stored at 2-8°C. Do not freeze.

Country of Origin

USA

Shipping

Next Day 2-8°C

Excitation Laser

Yellow Laser (563 nm)

RRID

AB_2828835

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Human Caspase-3 Active Subunit (p17) (CASP3) (Clone C31.129) recognizes an epitope on Human CASP3. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background

Caspase-3 is a member of the caspase family of cysteine proteases and plays a key role in mediating Fas (CD95) and tumor necrosis factor–1 (TNFR-1) induced apoptosis. Caspases can be divided into three groups: apoptotic initiators, apoptotic executioners, and inflammatory mediators.^{1, 2} Caspase-3 is activated during apoptotic signaling events by upstream proteases including caspase 6 and caspase 8.³ Caspase-3 is thought to be essential for chromatin margination, DNA fragmentation and nuclear collapse during apoptosis.³

Antigen Details

Protein

Caspase-3

PubMed

Caspase-3

NCBI Gene Bank ID

836

UniProt.org

Information on Uniprot.org

References & Citations

1. Adadah, BA. et al. (2000) Journal of Neurotoma 17(10): 811
2. Salvesen, GS. et al. (1999) Proc. Natl. Acad. Sci. USA 96(20): 10964
3. Slee, EA. et al. (2001) J. Biol. Chem. 276(10):7320

Technical Protocols